Identification of porcine CTLA4 gene polymorphism and their association with piglet diarrhea and performance traits

被引:0
|
作者
Gao, Xiaowen [1 ]
Guo, Dongchun [2 ]
Kou, Mingxing [1 ]
Xing, Guiling [1 ]
Zha, Andong [1 ]
Yang, Xiuqin [1 ]
Wang, Xibiao [1 ]
Di, Shengwei [1 ]
Cai, Jiancheng [3 ]
Niu, Buyue [1 ]
机构
[1] Northeast Agr Univ, Coll Anim Sci & Technol, Harbin 150030, Heilongjiang, Peoples R China
[2] CAAS, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Harbin 150001, Heilongjiang, Peoples R China
[3] Lanxi Breeding Farm, Lanxi 151500, Peoples R China
基金
中国国家自然科学基金;
关键词
Pig; CTLA4; gene; Polymorphism; Haplotype; Diarrhea; CANCER RISK; LARGE WHITE; ANTIGEN; EXPRESSION; LANDRACE; BLOOD;
D O I
10.1007/s11033-018-4536-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The objective of this study was to evaluate the association between the cytotoxic T-lymphocyte-associated antigen 4 (CTLA4) gene and piglet diarrhea. In this study, the mRNA expression of the CTLA4 gene increased significantly in IPEC-J2 cells after Escherichia coli K88 infection. Single nucleotide polymorphisms (SNPs) located in the 5 flanking region (SNPs g.107281989C>T) and 3-untranslated region (3-UTR; SNPs g.107288753C>A) were identified, and they were in linkage disequilibrium in both Min pigs and the Landrace population. Association analysis showed that Landrace piglets with a TT or AA genotype had a lower diarrhea index, and AA animals had higher average daily gain when compared to CC pigs, respectively (p<0.05). However, the relationship between SNPs and diarrhea and performance traits in the Min population was not significant. Haplotype analysis indicated that the TC haplotype had the lowest diarrhea index. The 5 flanking deletion assay suggested that SNP g.107281989C>T was a molecular marker instead of the functional marker. This research demonstrated that genetic variances in the CTLA4 gene had significant effects on Landrace piglet diarrhea resistance.
引用
收藏
页码:813 / 822
页数:10
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