Phosphorylation-Dependent SUMOylation of the Transcription Factor NF-E2

被引：11

作者：

Su, Yee-Fun ^{[1
]}

Shyu, Yu-Chiau ^{[3
,4
]}

Shen, Che-Kun James ^{[1
]}

Hwang, Jaulang ^{[1
,2
]}

机构：

[1] Acad Sinica, Inst Mol Biol, Taipei, Taiwan

[2] Taipei Med Univ, Sch Med, Dept Biochem, Taipei, Taiwan

[3] Natl Yang Ming Univ, Inst Biopharmaceut Sci, Taipei 112, Taiwan

[4] Taipei City Hosp, Dept Educ & Res, Taipei, Taiwan

来源：

PLOS ONE | 2012年 / 7卷 / 09期

关键词：

ERYTHROLEUKEMIA CELL-DIFFERENTIATION; LEUCINE-ZIPPER PROTEIN; SUMO MODIFICATION; GENE-EXPRESSION; TOPOISOMERASE-I; REPRESSION; PATHWAYS; KINASE; JUN;

D O I：

10.1371/journal.pone.0044608

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Nuclear factor erythroid-derived 2 (NF-E2), a heterodimer composed of p45 and p18, is a transcriptional activator in hematopoietic progenitors. The transcriptional activity of NF-E2 is not only upregulated by SUMOylation but also stimulated by the cAMP-dependent protein kinase A (PKA). However, the relationship between SUMOylation and phosphorylation in the activation of NF-E2 is unclear. In the present studies, we have demonstrated that PKA enhances NF-E2 SUMOylation in an in vitro system using purified proteins, suggesting a possible mechanism for PKA-dependent activation of the NF-E2 transcription factor through SUMOylation.

引用

页数：7

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