One-pot intramolecular cyclization of 5-hydroxymethylcytosine for sequencing DNA hydroxymethylation at single-base resolution

被引：2

作者：

Ma, Yangde ^{[1
,2
]}

Zhang, Ning ^{[2
]}

Chen, Shaokun ^{[1
,2
]}

Sun, Jingwei ^{[1
]}

Liu, Yan ^{[1
,2
]}

Li, Xiangjun ^{[1
]}

Wang, Hailin ^{[1
,2
,3
]}

机构：

[1] Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China

[2] Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, 18 Shuangqing Rd, Beijing 100085, Peoples R China

[3] Jianghan Univ, Inst Environm & Hlth, 8 Sanjiaohu Rd, Wuhan 430056, Hubei, Peoples R China

来源：

ANALYST | 2021年 / 146卷 / 03期

基金：

中国国家自然科学基金;

关键词：

D O I：

10.1039/d0an01981j

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Here we establish a one-pot reaction to directly convert the DNA base 5-hydroxymethylcytosine (5hmC) to an intramolecular cyclization nucleobase, which loses both protons of the exocyclic N4-amino group and thus is recognized as thymine (T) by DNA polymerase. Based on this 5hmC-specific reaction, a prospective bisulfite-free strategy for 5hmC sequencing is proposed. This is also the first example to show modified DNA labeling in non-water solvent-dominant media for DNA sequencing.

引用

页码：820 / 824

页数：5

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