Comparison of Chondrogenic Potential in Equine Mesenchymal Stromal Cells Derived from Adipose Tissue and Bone Marrow

被引:148
|
作者
Vidal, Martin A.
Robinson, Sandra O.
Lopez, Mandi J.
Paulsen, Daniel B.
Borkhsenious, Olga
Johnson, Jill R.
Moore, Rustin M.
Gimble, Jeffrey M.
机构
[1] Louisiana State Univ, Dept Vet Clin Sci, Equine Hlth Studies Program, Lab Equine & Comparat Orthoped Res, Baton Rouge, LA 70803 USA
[2] Louisiana State Univ, Sch Vet Med, Dept Pathobiol Sci, Baton Rouge, LA 70803 USA
[3] Louisiana State Univ, Sch Vet Med, Dept Comparat Biomed Sci, Baton Rouge, LA 70803 USA
[4] Ohio State Univ, Coll Vet Med, Dept Vet Clin Sci, Columbus, OH 43210 USA
[5] Louisiana State Univ, Pennington Biomed Res Ctr, Stem Cell Lab, Baton Rouge, LA 70808 USA
关键词
D O I
10.1111/j.1532-950X.2008.00462.x
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
To compare the chondrogenic potential of adult equine mesenchymal stem cells derived from bone marrow (MSCs) or adipose tissue (ASCs). In vitro experimental study. Adult Thoroughbred horses (n=11). BM (5 horses; mean [+/- SD] age, 4 +/- 1.4 years) or adipose tissue (6 horses; mean age, 3.5 +/- 1.1 years) samples were obtained. Cryopreserved MSCs and ASCs were used for pellet cultures in stromal medium (C) or induced into chondrogenesis +/- transforming growth factor-3 (TGF beta(3)) and bone morphogenic factor-6 (BMP-6). Pellets harvested after 3, 7, 14, and 21 days were examined for cross-sectional size and tissue composition (hematoxylin and eosin), glycosaminoglycan (GAG) staining (Alcian blue), collagen type II immunohistochemistry, and by transmission electron microscopy. Pellet GAG and total DNA content were measured using dimethylmethylene blue and Hoechst DNA assays. Collagen type II synthesis was predominantly observed in MSC pellets from Day 7 onward. Unlike ASC cultures, MSC pellets had hyaline-like matrix by Day 14. GAG deposition occurred earlier in MSC cultures compared with ASC cultures and growth factors enhanced both MSC GAG concentrations (P <.0001) and MSC pellet size (P <.004) after 2 weeks in culture. Equine MSCs have superior chondrogenic potential compared with ASCs and the equine ASC growth factor response suggests possible differences compared with other species. Elucidation of equine ASC and MSC receptor profiles will enhance the use of these cells in regenerative cartilage repair.
引用
收藏
页码:713 / 724
页数:12
相关论文
共 50 条
  • [11] Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow-Derived Cells in Osteoarthritic In Vitro Models
    Pagani, Stefania
    Borsari, Veronica
    Veronesi, Francesca
    Ferrari, Andrea
    Cepollaro, Simona
    Torricelli, Paola
    Filardo, Giuseppe
    Fini, Milena
    JOURNAL OF CELLULAR PHYSIOLOGY, 2017, 232 (06) : 1478 - 1488
  • [12] Proliferative and chondrogenic potential of mesenchymal stromal cells from pluripotent and bone marrow cells
    Sfougataki, Irene
    Varela, Ioanna
    Stefanaki, Kalliope
    Karagiannidou, Angeliki
    Roubelakis, Maria G.
    Kalodimou, Vasiliki
    Papathanasiou, Ioanna
    Traeger-Synodinos, Joanne
    Kitsiou-Tzeli, Sofia
    Kanavakis, Emmanuel
    Kitra, Vasiliki
    Tsezou, Aspasia
    Tzetis, Maria
    Goussetis, Evgenios
    HISTOLOGY AND HISTOPATHOLOGY, 2020, 35 (12) : 1415 - 1426
  • [13] Comparison of Chondrogenic Differentiation of Adipose Tissue-Derived Mesenchymal Stem Cells with Cultured Chondrocytes and Bone Marrow Mesenchymal Stem Cells
    Havlas, V.
    Kos, P.
    Jendelova, P.
    Lesny, P.
    Trc, T.
    Sykova, E.
    ACTA CHIRURGIAE ORTHOPAEDICAE ET TRAUMATOLOGIAE CECHOSLOVACA, 2011, 78 (02) : 138 - 144
  • [14] POTENTIAL OF MESENCHYMAL STROMAL CELLS DERIVED FROM HUMAN ADULT ADIPOSE TISSUE AND BONE MARROW FOR REGENERATIVE MEDICINE AND TISSUE ENGINEERING
    Petrenko, Y. A.
    Skorobogatova, N. G.
    Petrenko, A. Y.
    INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS, 2011, 34 (08): : 690 - 690
  • [15] Chondrogenic potential of progenitor cells derived from human bone marrow and adipose tissue: A patient-matched comparison
    Huang, JI
    Kazmi, N
    Durbhakula, MM
    Hering, TM
    Yoo, JU
    Johnstone, B
    JOURNAL OF ORTHOPAEDIC RESEARCH, 2005, 23 (06) : 1383 - 1389
  • [16] Comparison of mesenchymal stem cells derived from bone marrow and adipose tissue of minipigs
    Peterbauer, A.
    Schauer, K.
    Neussl, S.
    Schultz, A.
    Gabriel, C.
    Redl, H.
    van Griensven, M.
    Wolbank, S.
    TISSUE ENGINEERING PART A, 2008, 14 (05) : 828 - 828
  • [17] Biological Comparison of Mesenchymal Stem Cells Derived from Bone Marrow and Adipose Tissue
    Izadpanah, Reza
    Trygg, Cynthia
    Kriedt, Christopher
    Bunnell, Bruce
    MOLECULAR THERAPY, 2006, 13 : S134 - S135
  • [18] Characterization and osteogenic potential of equine muscle tissue- and periosteal tissue-derived mesenchymal stem cells in comparison with bone marrow- and adipose tissue derived mesenchymal stem cells
    Radtke, Catherine L.
    Nino-Fong, Rodolfo
    Gonzalez, Blanca P. Esparza
    Stryhn, Henrik
    McDuffee, Laurie A.
    AMERICAN JOURNAL OF VETERINARY RESEARCH, 2013, 74 (05) : 790 - 800
  • [19] Equine Bone Marrow and Adipose Tissue Mesenchymal Stem/Stromal Cells Putatively Derive from Perivascular Stem Cells
    Esteves, C. L.
    Sheldrake, T.
    Sawson, L.
    Menghini, T.
    Khan, N.
    Peault, B.
    Donadeu, F. X.
    TISSUE ENGINEERING PART A, 2015, 21 : S48 - S49
  • [20] Microvascular Networks From Endothelial Cells and Mesenchymal Stromal Cells From Adipose Tissue and Bone Marrow: A Comparison
    Pill, Karoline
    Melke, Johanna
    Muehleder, Severin
    Pultar, Marianne
    Rohringer, Sabrina
    Priglinger, Eleni
    Redl, Heinz R.
    Hofmann, Sandra
    Holnthoner, Wolfgang
    FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, 2018, 6