Quantifying protein dynamics and stability in a living organism

被引：41
|
作者：
Feng, Ruopei [1 ]
Gruebele, Martin [1 ,2 ,3 ]
Davis, Caitlin M. [1 ,2 ]
机构：
[1] Univ Illinois, Dept Chem, Urbana, IL 61801 USA
[2] Univ Illinois, Dept Phys, Urbana, IL 61801 USA
[3] Univ Illinois, Ctr Biophys & Quantitat Biol, Urbana, IL 61801 USA
来源：
NATURE COMMUNICATIONS | 2019年 / 10卷 / 1期
基金：
美国国家科学基金会;
关键词：
EXCLUDED-VOLUME; TRANSGENESIS; CRYSTALLIN; GENE;
D O I：
10.1038/s41467-019-09088-y
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
As an integral part of modern cell biology, fluorescence microscopy enables quantification of the stability and dynamics of fluorescence-labeled biomolecules inside cultured cells. However, obtaining time-resolved data from individual cells within a live vertebrate organism remains challenging. Here we demonstrate a customized pipeline that integrates meganuclease-mediated mosaic transformation with fluorescence-detected temperaturejump microscopy to probe dynamics and stability of endogenously expressed proteins in different tissues of living multicellular organisms.
引用
收藏
页数：7
相关论文
共 50 条
  • [31] Studying protein dynamics in living cells
    Jennifer Lippincott-Schwartz
    Erik Snapp
    Anne Kenworthy
    Nature Reviews Molecular Cell Biology, 2001, 2 : 444 - 456
  • [32] Landscape and flux for quantifying global stability and dynamics of game theory
    Xu, Li
    Wang, Jin
    PLOS ONE, 2018, 13 (08):
  • [33] Protein stability imposes limits on organism complexity and speed of molecular evolution
    Zeldovich, Konstantin B.
    Chen, Peiqiu
    Shakhnovich, Eugene I.
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (41) : 16152 - 16157
  • [34] Leibniz, the living being and organism
    Malaterre, Christophe
    HISTORY AND PHILOSOPHY OF THE LIFE SCIENCES, 2011, 33 (03): : 406 - 407
  • [35] Leibniz. The living and the organism
    Perru, Olivier
    REVUE PHILOSOPHIQUE DE LOUVAIN, 2012, 110 (03) : 545 - 548
  • [36] The living organism as a biocybernetic unity
    Schjelderup, V
    PROCEEDINGS OF THE 3RD INTERNATIONAL CONGRESS OF ECIWO BIOLOGY, 1996, : 458 - 464
  • [37] The Largest and Oldest Living Organism
    Maheshwari, Ramesh
    RESONANCE-JOURNAL OF SCIENCE EDUCATION, 2005, 10 (04): : 4 - 9
  • [38] The largest and oldest living organism
    Ramesh Maheshwari
    Resonance, 2005, 10 (4) : 4 - 9
  • [39] The private diary as a living organism
    Lejeune, P
    NOUVELLE REVUE FRANCAISE, 1997, (531): : 53 - 63
  • [40] The living organism: Strengthening the basis
    Cazalis, Roland
    Carletti, Timoteo
    Cottam, Ron
    BIOSYSTEMS, 2017, 158 : 10 - 16
12345