A multiplex PCR for 11 X chromosome STR markers and population data from a Brazilian Amazon Region

被引：27
|
作者：
Ribeiro Rodrigues, Elzemar Martins
das Neves Leite, Fabio Pereira [2 ,3 ]
Hutz, Mara Helena [3 ]
Ferreira Palha, Teresinha de Jesus Brabo [4 ]
Campos Ribeiro dos Santos, Andrea Kely
Batista dos Santos, Sidney Emanuel [1 ]
机构：
[1] Fed Univ Para, Ctr Ciencias Biol, Lab Genet Humana & Med, Dept Pathol, BR-66075970 Belem, Para, Brazil
[2] Inst Geral Pericias, Lab Pericias, Setor Biol Mol, Porto Alegre, RS, Brazil
[3] Univ Fed Rio Grande do Sul, Dept Genet, Porto Alegre, RS, Brazil
[4] Ctr Pericias Cient Renato Chaves, Porto Alegre, RS, Brazil
来源：
FORENSIC SCIENCE INTERNATIONAL-GENETICS | 2008年 / 2卷 / 02期
关键词：
Amazon; Multiplex PCR; X-STR markers; Population data; Forensic genetic;
D O I：
10.1016/j.fsigen.2007.10.179
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The analysis of X-STR polymorphisms has received the attention of several researchers, mainly due to its applicability to the investigation of complex kinship cases. Although many X-STRs have been validated for forensic use, little is known about the variations of these polymorphisms in different populations of the world. The present work describes a new multiplex system that allows the simultaneous analysis of 11 X-STR markers, for use both in paternity determination and more complex forensic cases. The loci investigated include DXS9895, DXS7132, DXS6800, DXS9898, DXS6789, DXS7133, DXS7130, HPRTB, GATA31E08, DXS7423, and DXS10011. which together afford a power of discrimination in the order of 0.999999. In addition, this work presents the genotyping results obtained for a sample of 324 individuals (182 males and 142 females) from the admixed population of Belem, Para, located in the Brazilian Amazon Region. (C) 2007 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：154 / 158
页数：5
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