Improvement of the Lipophilic-Oxygen Radical Absorbance Capacity (L-ORAC) Method and Single-Laboratory Validation

被引：18

作者：

Watanabe, Jun ^{[1
]}

Oki, Tomoyuki ^{[2
]}

Takebayashi, Jun ^{[3
]}

Yamasaki, Koji ^{[4
]}

Takano-Ishikawa, Yuko ^{[1
]}

Hino, Akihiro ^{[1
]}

Yasui, Akemi ^{[1
]}

机构：

[1] Natl Agr & Food Res Org, Natl Food Res Inst, Tsukuba, Ibaraki 3058642, Japan

[2] Natl Agr & Food Res Org, Kyushu Okinawa Agr Res Ctr, Kumamoto 8611192, Japan

[3] Natl Inst Hlth & Nutr, Shinjuku Ku, Tokyo 1628636, Japan

[4] Taiyo Kagaku Co, Yokaichi, Mie 5100844, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2013年 / 77卷 / 04期

关键词：

lipophilic oxygen radical absorbance capacity; antioxidant capacity; method validation; ANTIOXIDANT CAPACITY; ASSAY;

D O I：

10.1271/bbb.120786

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We improved the procedure for lipophilic-oxygen radical absorbance capacity (L-ORAC) measurement for better repeatability and intermediate precision. A sealing film was placed on the assay plate, and glass vials and microdispensers equipped with glass capillaries were used. The antioxidant capacities of food extracts can be evaluated by this method with nearly the same precision as antioxidant solutions.

引用

页码：857 / 859

页数：3