Preparation of nano-scaled magnetic biological probes of Fe3O4/dextran/antibody and chromatographic assay

被引:0
|
作者
Hong, X
Guo, W
Li, J
Liu, YM
Yang, WS
Bai, YB [1 ]
Li, TJ
Yuan, H
Ma, L
机构
[1] Jilin Univ, Coll Chem, Changchun 130023, Peoples R China
[2] Yunnan Univ, Monoclonal Antibody Technol Ctr, Kunming 650091, Peoples R China
来源
关键词
magnetic biological probes; antigen-antibody recognition; BSA blocking; chromatographic assay;
D O I
暂无
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Dextran-coated magnetic nanocomposites were prepared. A periodate oxidation method was used to further activate the magnetic dextran, forming magnetic polyaldehyde-dextran, which could be conjugated to biomolecules, antibodes. The magnetic labeled nano-scaled bioconjugates could be well blocked with BSA and exhibit high activity by ELISA and immunochromatographic strip assay.
引用
收藏
页码:445 / 447
页数:3
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