ANALYSIS OF CYTOPLASMIC MRNA DECAY IN SACCHAROMYCES CEREVISIAE

被引：15
|
作者：
Passos, Dario O. [1 ]
Parker, Roy
机构：
[1] Univ Arizona, Dept Mol & Cellular Biol, Tucson, AZ 85721 USA
来源：
RNA TURNOVER IN EUKARYOTES: NUCLEASES, PATHWAYS AND ANAYLSIS OF MRNA DECAY | 2008年 / 448卷
关键词：
D O I：
10.1016/S0076-6879(08)02620-7
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The yeast, Saccharomyces cerevisiae, is a model system for the study of eukaryotic mRNA degradation. In this organism, a variety of methods have been developed to measure mRNA decay rates, trap intermediates in the mRNA degradation process, and establish precursor-product relationships. In addition, the use of mutant strains lacking specific enzymes involved in mRNA destruction, or key regulatory proteins, allows one to determine the mechanisms by which individual mRNAs are degraded. In this chapter, we discuss methods for analyzing mRNA degradation in S. cerevisiae.
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页码：409 / 427
页数：19
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