UPARSE: highly accurate OTU sequences from microbial amplicon reads

被引：487
|
作者：
Edgar, Robert C.
机构：
[1] Independent Investigator,
来源：
NATURE METHODS | 2013年 / 10卷 / 10期
关键词：
DIVERSITY; DATABASE;
D O I：
10.1038/NMETH.2604
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Amplified marker-gene sequences can be used to understand microbial community structure, but they suffer from a high level of sequencing and amplification artifacts. The UPARSE pipeline reports operational taxonomic unit (OTU) sequences with <= 1% incorrect bases in artificial microbial community tests, compared with >3% incorrect bases commonly reported by other methods. The improved accuracy results in far fewer OTUs, consistently closer to the expected number of species in a community.
引用
收藏
页码：996 / +
页数：5
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