Inhibitory effects of Schisandra chinensis extract on acne-related inflammation and UVB-induced photoageing

Context: Schisandra chinensis (Turcz.) Baill. (Schisandraceae) fruit extract (SFE) has been reported to induce non-specific tissue protection against inflammation in vivo. However, the effects of SFE on Propionibacterium acnes-stimulated acne and UVB-irradiated photoageing have yet to be investigated. Objective: To systematically investigate the effects of SFE against P. acnes and photoageing in vitro. Materials and methods: Qualitative and quantitative analyses of SFE were performed by HPLC. SFE concentrations from 2.5 to 50 mu g/mL were tested. Specifically, ELISA was used to examine the levels of proinflammatory cytokines in THP-1 cells as well as of collagen I and matrix metalloproteinases-1 in HDF cells. The anti-bacterial effect of SFE was determined using the microdilution broth method. Glutathione and malondialdehyde levels were examined using the colorimetric and TBA methods, respectively. The degree of ageing was determined by cytochemical staining. Results: SFE significantly inhibited P. acnes growth (MIC 0.5mg/mL) and 50 mu g/mL of SFE suppressed the production of interleukin-1 beta, interleukin-8 and tumour necrosis factor alpha, by 59.67%, 62.69% and 68.30%, respectively, in P. acnes-stimulated THP-1 cells. Additionally, 10 mu g/mL of SFE suppressed photoageing in UVB-exposed fibroblasts by decreasing metalloproteinase levels by 88.4%, inducing collagen by 58.4% and activating the anti-oxidant defence system, by limiting lipid peroxidation by 51.1% and increasing glutathione production by 34.1% (2.5 mu g/mL SFE). Discussion and conclusion: These results indicated that SFE could significantly ameliorate the inflammatory state in P. acnes-stimulated THP-1 and UVB-irradiated HDF cells, suggesting its potential as a novel agent for acne therapy and photoageing prevention.