Production of novel NaN3-resistant creatine amidinohydrolase in recombinant Escherichia coli (vol 6, pg 248, 2015)

被引：3

作者：

Liu, Song ^{[1
,2
,3
]}

Dai, Jun ^{[1
,2
,3
]}

Kang, Zhen ^{[1
,2
,3
]}

Li, Jianghua ^{[1
,2
,3
]}

Chen, Jian ^{[2
,3
]}

Du, Guocheng ^{[2
,3
]}

机构：

[1] Jiangnan Univ, Minist Educ, Key Lab Ind Biotechnol, Wuxi, Peoples R China

[2] Jiangnan Univ, Sch Biotechnol, Wuxi, Peoples R China

[3] Jiangnan Univ, Synerget Innovat Ctr Food Safety & Nutr, Wuxi, Jiangsu, Peoples R China

来源：

BIOENGINEERED | 2015年 / 6卷 / 04期

关键词：

arthrobacter nicotianae; creatinase; escherichia coli; NaN3-resistance; HIGH-LEVEL SECRETION; PSEUDOMONAS-PUTIDA; CONSTRUCTION; PURIFICATION; EXPRESSION; CLONING; SYSTEM; GENE;

D O I：

10.1080/21655979.2015.1052919

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Creatinase (creatine amidinohydrolase), an important medical enzyme, has been used for clinical diagnosis of renal function because of its high substrate specificity. Recently, we successfully cloned a NaN3-resistant creatinase encoding gene from Arthrobacter nicotianae. By optimizing the cultivation process, we realized its high-level expression in Escherichia coli. In this addendum, production of this NaN3-resistant creatinase in Escherichia coli and future research were further discussed.

引用

页码：248 / 250

页数：3