Contribution of PCR Denaturing Gradient Gel Electrophoresis Combined with Mixed Chromatogram Software Separation for Complex Urinary Sample Analysis

被引：3

作者：

Kotaskova, Iva ^{[1
]}

Malisova, Barbora ^{[1
]}

Obrucova, Hana ^{[1
]}

Hola, Veronika ^{[2
]}

Peroutkova, Tereza ^{[2
]}

Ruzicka, Filip ^{[2
]}

Freiberger, Tomas ^{[1
,3
,4
]}

机构：

[1] Masaryk Univ, Fac Med, Ctr Cardiovasc Surg & Transplantat, Mol Genet Lab, Brno, Czech Republic

[2] Masaryk Univ, Fac Med, Dept Microbiol, Brno, Czech Republic

[3] Masaryk Univ, Fac Med, Dept Clin Immunol & Allergol, Brno, Czech Republic

[4] St Annes Univ Hosp, Brno, Czech Republic

来源：

JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY | 2017年 / 27卷 / 06期

关键词：

Complex sample; Mixed chromatogram; PCR denaturing gradient gel electrophoresis; RipSeq Mixed; Catheter; DIVERSITY;

D O I：

10.1159/000484524

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Complex samples are a challenge for sequencing-based broad-range diagnostics. We analysed 19 urinary catheter, ureteral Double-J catheter, and urine samples using 3 methodological approaches. Out of the total 84 operational taxonomic units, 37, 61, and 88% were identified by culture, PCR-DGGE-SS (PCR denaturing gradient gel electrophoresis followed by Sanger sequencing), and PCR-DGGE-RM (PCR-DGGE combined with software chromatogram separation by RipSeq Mixed tool), respectively. The latter approach was shown to be an efficient tool to complement culture in complex sample assessment. (C) 2017 S. Karger AG, Basel

引用

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页码：350 / 355

页数：6

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