Robust, synergistic regulation of human gene expression using TALE activators

被引：0

作者：

Maeder, Morgan L. ^{[1
,2
,3
,4
]}

Linder, Samantha J. ^{[1
,2
,3
]}

Reyon, Deepak ^{[1
,2
,3
,5
]}

Angstman, James F. ^{[1
,2
,3
]}

Fu, Yanfang ^{[1
,2
,3
,5
]}

Sander, Jeffry D. ^{[1
,2
,3
,5
]}

Joung, J. Keith ^{[1
,2
,3
,4
,5
]}

机构：

[1] Massachusetts Gen Hosp, Mol Pathol Unit, Charlestown, MA 02129 USA

[2] Massachusetts Gen Hosp, Ctr Computat & Integrat Biol, Charlestown, MA USA

[3] Massachusetts Gen Hosp, Ctr Canc Res, Charlestown, MA USA

[4] Harvard Univ, Sch Med, Program Biol & Biomed Sci, Boston, MA USA

[5] Harvard Univ, Sch Med, Dept Pathol, Boston, MA 02115 USA

来源：

NATURE METHODS | 2013年 / 10卷 / 03期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

TRANSCRIPTION; PROTEINS; EFFECTORS; CELLS; RNAI;

D O I：

10.1038/NMETH.2366

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Artificial activators designed using transcription activator like effector (TALE) technology have broad utility, but previous studies suggest that these monomeric proteins often exhibit low activities. Here we demonstrate that TALE activators can robustly function individually or in synergistic combinations to increase expression of endogenous human genes over wide dynamic ranges. These findings will encourage applications of TALE activators for research and therapy, and guide design of monomeric TALE-based fusion proteins.

引用

页码：243 / 245

页数：3

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