Detection of Peronophythora litchii on lychee by loop-mediated isothermal amplification assay

被引：7
|
作者：
Kong, Guanghui [1 ]
Li, Tinglu [1 ]
Huang, Weixiong [1 ]
Li, Minhui [1 ]
Shen, Wankuan [2 ]
Jiang, Liqun [1 ,4 ]
Hsiang, Tom [3 ]
Jiang, Zide [1 ]
Xi, Pinggen [1 ,5 ]
机构：
[1] South China Agr Univ, Guangdong Prov Key Lab Microbial Signals & Dis Co, Dept Plant Pathol, Guangzhou 510642, Peoples R China
[2] South China Agr Univ, Sci Observing & Expt Stn Crop Cultivat South Chin, Coll Agron, Guangzhou 510642, Peoples R China
[3] Univ Guelph, Sch Environm Sci, Guelph, ON N1G 2W1, Canada
[4] Guangdong Acad Agr Sci, Rice Res Inst, Guangdong Prov Key Lab New Technol Rice Breeding, Guangzhou, Peoples R China
[5] Guangdong Lab Lingnan Modem Agr, Maoming Branch, Guangzhou, Peoples R China
来源：
CROP PROTECTION | 2021年 / 139卷
关键词：
Peronophythora litchii; Lychee; Loop-mediated isothermal amplification; Nested PCR; RAPID DETECTION; SENSITIVE DETECTION; LAMP; FRUIT; PCR;
D O I：
10.1016/j.cropro.2020.105370
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Downy blight caused by Peronophythora litchii is one of the most destructive diseases of lychee (Litchi chinensis Sonn.). Early diagnosis of the P. litchii pathogen should be the top priority for addressing disease epidemics and management. In this study, loop-mediated isothermal amplification (LAMP) assays targeting the M90 gene sequences were developed for specific detection of P. litchii. The detection limit of LAMP assay was 10 pg genomic DNA of P. litchii in 25 mu L reaction mixtures. LAMP assay and nested polymerase chain reaction (PCR) assay showed higher sensitivity than conventional PCR. The LAMP assay could also detect the presence of P. litchii in infected leaves, fruits and soils. This study provides a simple, highly sensitive, rapid and reliable protocol for the early diagnosis of P. litchii on lychee.
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页数：6
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