Development of Loop-Mediated Isothermal Amplification Assay for Detection of Entamoeba histolytica

被引：52

作者：

Liang, Shih-Yu ^{[2
]}

Chan, Yun-Hsien ^{[1
]}

Hsia, Kan-Tai ^{[2
]}

Lee, Jing-Lun ^{[1
]}

Kuo, Ming-Chu ^{[1
]}

Hwa, Kuo-Yuan ^{[3
]}

Chan, Chi-Wen ^{[1
]}

Chiang, Ting-Yi ^{[1
]}

Chen, Jung-Sheng ^{[1
]}

Wu, Fang-Tzy ^{[1
]}

Ji, Dar-Der ^{[1
]}

机构：

[1] Ctr Dis Control, Res & Diagnost Ctr, Taipei, Taiwan

[2] Natl Yang Ming Univ, Inst Oral Biol, Sch Dent, Taipei 112, Taiwan

[3] Natl Taipei Univ Technol, Dept Mol Sci & Engn, Taipei, Taiwan

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2009年 / 47卷 / 06期

关键词：

ANTIGEN-DETECTION; PCR ASSAY; DIAGNOSIS; INFECTION; AMEBIASIS; DNA; DISPAR; DIFFERENTIATION; SAMPLES; FECES;

D O I：

10.1128/JCM.00105-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A novel one-step, closed-tube, loop-mediated isothermal amplification (LAMP) assay for detecting Entamoeba histolytica, one of the leading causes of morbidity in developing countries, was developed. The sensitivity of the LAMP assay is 1 parasite per reaction. A total of 130 clinical samples were analyzed, and the results compared with those of conventional nested PCR to validate the practicability of this assay. No DNA was amplified from other diarrheal pathogens, such as other Entamoeba species, bacteria, and viruses. These results indicate that LAMP is a rapid, simple, and valuable diagnostic tool for epidemiological studies of amebiasis.

引用

页码：1892 / 1895

页数：4

共 50 条

[1] Development of loop-mediated isothermal amplification for rapid detection of Entamoeba histolytica
Rivera, Windell L.
Ong, Vanissa A.
[J]. ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE, 2013, 6 (06) : 457 - 461
[2] Development of loop-mediated isothermal amplification for rapid detection of Entamoeba histolytica
Windell L.Rivera
Vanissa A.Ong
[J]. Asian Pacific Journal of Tropical Medicine, 2013, (06) : 457 - 461
[3] Loop-mediated isothermal amplification (LAMP) assay for rapid detection of Entamoeba histolytica in amoebic liver abscess
Singh, Preeti
Mirdha, Bijay Ranjan
Ahuja, Vineet
Singh, Sundeep
[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2013, 29 (01): : 27 - 32
[4] A rapid multiplex loop-mediated isothermal amplification (mLAMP) assay for detection of Entamoeba histolytica and Giardia duodenalis
Mewara, Abhishek
Khunger, Sandhya
Sharma, Chayan
Krishnamoorthi, Sivanantham
Singh, Shreya
Yadav, Rakesh
Khurana, Sumeeta
Sehgal, Rakesh
[J]. LETTERS IN APPLIED MICROBIOLOGY, 2023, 76 (10)
[5] Loop-mediated isothermal amplification (LAMP) assay for rapid detection of Entamoeba histolytica in amoebic liver abscess
Preeti Singh
Bijay Ranjan Mirdha
Vineet Ahuja
Sundeep Singh
[J]. World Journal of Microbiology and Biotechnology, 2013, 29 : 27 - 32
[6] Development of a loop-mediated isothermal amplification assay for the detection of porcine hokovirus
Li, Bin
Sun, Bing
Du, Lu-ping
Mao, Ai-hua
Wen, Li-bin
Ni, Yan-xiu
Zhang, Xue-han
He, Kong-wang
[J]. JOURNAL OF VIROLOGICAL METHODS, 2013, 193 (02) : 415 - 418
[7] Development of a loop-mediated isothermal amplification assay for the detection of Mycobacterium bovis
Zhang, Jun
Zhang, Gui-Hong
Yang, Lin
Huang, Ren
Zhang, Yu
Jia, Kun
Yuan, Wen
Li, Shou-Jun
[J]. VETERINARY JOURNAL, 2011, 187 (03): : 393 - 396
[8] Development of a loop-mediated isothermal amplification assay for the detection of Harpophora maydis
Gonzalez, N.
Munkvold, G.
[J]. PHYTOPATHOLOGY, 2016, 106 (12) : 111 - 111
[9] Development of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Capripoxviruses
Das, Amaresh
Babiuk, Shawn
McIntosh, Michael T.
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2012, 50 (05) : 1613 - 1620
[10] Development of a loop-mediated isothermal amplification assay for detection of Phytophthora sojae
Dai, Ting-Ting
Lu, Chen-Chen
Lu, Jing
Dong, SuoMeng
Ye, WenWu
Wang, YuanChao
Zheng, XiaoBo
[J]. FEMS MICROBIOLOGY LETTERS, 2012, 334 (01) : 27 - 34

← 1 2 3 4 5 →