Quantum dot-based lateral flow immunoassay for detection of chloramphenicol in milk

被引：166

作者：

Berlina, Anna N. ^{[1
]}

Taranova, Nadezhda A. ^{[1
]}

Zherdev, Anatoly V. ^{[1
]}

Vengerov, Yuri Y. ^{[1
]}

Dzantiev, Boris B. ^{[1
]}

机构：

[1] Russian Acad Sci, AN Bah Inst Biochem, Moscow 119071, Russia

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2013年 / 405卷 / 14期

关键词：

Chloramphenicol; Milk; Quantum dots; Lateral flow assay; IMMUNOCHROMATOGRAPHIC ASSAY;

D O I：

10.1007/s00216-013-6876-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel rapid (20 min) fluorescent lateral flow test for chloramphenicol (CAP) detection in milk was developed. The chosen format is a binding-inhibition assay. Water-soluble quantum dots with an emission peak at 625 nm were applied as a label. Milk samples were diluted by 20 % with phosphate buffer to eliminate the matrix effect. The result of the assay could be seen by eye under UV light excitation or registered by a portable power-dependent photometer. The limit of CAP detection by the second approach is 0.2 ng/mL, and the limit of quantitation is 0.3 ng/mL.

引用

页码：4997 / 5000

页数：4

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