Analysis of Codon Usage Patterns of Tianfu Goose Interferon Alpha

Interferons(IFNs) are a large family of cytokines, belonging to glycoproteins. As for the antiviral and antineoplastic activity of IFNs, IFNs were widely studied. Based of recent gene sequence analyses, we found that codon usages were variation in different IFN genes. Codon usage in a sample of 5 genes from the interferon alpha has been analysed using multivariate statistical analysis. Goose and duck IFN-alpha gene shared the similar patterns, and differ from chicken IFN-alpha gene codon usage biases pattern. In other words, goose and duck had a much closer relationship than chicken. Asn(L), His(H), Ala(A) and Pro(P) were the preferred amino acids. RSCU values of 25 codons were greater than 1.0, and these codons had a much higher frequency of occurrence. In the accordance with the comparison, E. coli was much suitable for the expression of Tianfu goose IFN-alpha gene than Yeast and Human system. (C) 2011 Published by Elsevier Ltd. Selection and/or peer-review under responsibility of the Asia-Pacific Chemical, Biological & Environmental Engineering Society (APCBEES)