OPTIMIZATION AND VALIDATION OF HPLC-FLD METHOD FOR DETERMINATION OF AFLATOXIN B1 AND PRODUCTION OF THIS MYCOTOXIN BY ASPERGILLUS SECTION FLAVI ISOLATES

被引:0
|
作者
Jakabova, Silvia [1 ]
Barborakova, Zuzana [2 ]
Tancinova, Dana [2 ]
Maskova, Zuzana [2 ]
Golian, Jozef [1 ]
机构
[1] Slovak Univ Agr, Fac Biotechnol & Food Sci, Inst Food Sci, Tr A Hlinku 2, Nitra 94976, Slovakia
[2] Slovak Univ Agr, Fac Biotechnol & Food Sci, Inst Biotechnol, Tr A Hlinku 2, Nitra 94976, Slovakia
来源
JOURNAL OF MICROBIOLOGY BIOTECHNOLOGY AND FOOD SCIENCES | 2022年
关键词
aflatoxin B 1; HPLC-FLD; method validation; Aspergillus section Flavi; isolates; LIQUID-CHROMATOGRAPHY; BRAZIL NUTS; LC-MS/MS; B-1; GROWTH; MAIZE; M-1; B1; G1;
D O I
暂无
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
A high performance liquid chromatography coupled with fluorescence detection (HPLC-FLD) was evaluated for the monitoring of aflatoxin B1 (AFB1) production by Aspergillus section Flavi isolates, which were found in the commodities of Slovak origin (barley and tomatoes). Rapid validation of the method was performed and the method was applied for the determination of the mycotoxin. Based on the calculated validation characteristics, the method showed high sensitivity and reproducibility. The LOD and LOQ determined were 0.03 and 0.10 mu g.mL-1 which is fully acceptable for the determination of the AFB1 in isolates from plant commodities. The calibration graph was linear in the concentration range of 0.01- 10.0 mu g.mL-1. The optimized method was used for the analysis of the mycotoxin in three Aspergillus section Flavi isolates. The submerse cultivation was performed at 25 +/- 1 degrees C in yeast extract sucrose liquid medium and material was collected on the 7th, 14th and 21st day of cultivation. Simple sample extraction was carried out with use of ethyl acetate, and measurement was done with use of the mobile phase consisting of water and methanol by HPLC-FLD. Production of the AFB1 by isolates was ranging from <LOD (0.03 mu g. mL-1) to 49.38 mu g.mL-1 with the highest concentration on the 14th day of cultivation. The maximum level of AFB1 was achieved by the Isolate 1 (isolated from barley, Kolinany, 2018).
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页数:4
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