Telomerase Complex from Yeast Saccharomyces cerevisiae Contains a Biotinylated Component

被引：0
|
作者：
Shcherbakova, D. M. [1 ]
Zvereva, M. I. [1 ]
Dontsova, O. A. [1 ]
机构：
[1] Moscow MV Lomonosov State Univ, Dept Chem, Moscow, Russia
来源：
ACTA NATURAE | 2009年 / 1卷 / 02期
基金：
俄罗斯基础研究基金会;
关键词：
yeast telomerase; biotin; biotinylation; ACETYL-COA-CARBOXYLASE; CATALYTIC SUBUNIT; PROTEIN ARC1P; TRANSFER-RNA; AFFINITY; AVIDIN; ENZYME; CELLS; BINDS; TLC1;
D O I：
10.32607/20758251-2009-1-2-81-85
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Telomerase adds telomeric repeats to single-stranded DNA at the ends of the chromosomes. This enzyme is a ribonucleoprotein complex. Telomerase from yeast Saccharomyces cerevisiae consists of TLC1 RNA, which serves as a template for the synthesis of telomeric repeats, telomerase reverse transcriptase Est 2p, and a number of accessory proteins (Est 1p, Est 3p, Ku70/Ku80, and Sm-complex). We found that the yeast telomerase complex contains a biotinylated component. The telomerase fraction containing biotinylated protein is active in vitro and constitutes a small part of the total amount of active telomerase isolated from cells. We speculate about the nature of the biotinylated component.
引用
收藏
页码：81 / 85
页数：5
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