Molecular differentiation of two sibling species of the black fly Simulium vittatum (Diptera: Simullidae) based on random amplified polymorphic DNA

被引:0
|
作者
Duncan, GA
Adler, PH
Pruess, KP [1 ]
Powers, TO
机构
[1] Univ Nebraska, Dept Entomol, Lincoln, NE 68583 USA
[2] Nebraska Wesleyan Univ, Lincoln, NE 68504 USA
[3] Clemson Univ, Dept Entomol, Clemson, SC 29634 USA
[4] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA
关键词
Simulium vittatum; cytospecies; RAPD analysis; molecular markers; population structure;
D O I
10.1139/G03-144
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Larvae of the black fly morphospecies Simulium vittatum front Colorado, Montana, Nebraska, and New Hampshire were cytologically identified as either the IS-7 or the IIIL-1 cytospecies. DNA was PCR amplified from cytotyped larvae using eight 10-mer primers, labeled with 33 P, and run on polyacrylamide gels. The entire data set of 96 amplicons produced incomplete separation of the two cytospecies when subjected to neighbor-joining and maximum parsimony analyses. However, when analyzed within geographical regions, separate species status was supported. Bootstrap support for distinctness of the two cytospecies was best in Colorado where they were collected in true sympatry. The IS-7 cytospecies was more polymorphic in the western states, where it differed most from IIIL-1, which was most variable in the eastern states. The frequencies of the 17 most common amplicons in the two cytospecies were inversely correlated. A model of speciation derived from the molecular evidence suggests that IS-7 evolved in the west and spread eastward, whereas IIIL-1 later originated in the east and spread westward.
引用
收藏
页码:373 / 379
页数:7
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