Resolution and contrast enhancement in coherent anti-Stokes Raman-scattering microscopy

被引：40
|
作者：
Gasecka, Alicja [1 ,2 ]
Daradich, Amy [1 ,2 ]
Dehez, Harold [1 ,2 ]
Piche, Michel [2 ]
Cote, Daniel [1 ,2 ]
机构：
[1] Inst Univ Sante Mentale Quebec, Quebec City, PQ G1J 2G3, Canada
[2] Univ Laval, Ctr Dopt Photon & Laser, Quebec City, PQ G1V 0A6, Canada
来源：
OPTICS LETTERS | 2013年 / 38卷 / 21期
关键词：
EMISSION; BREAKING; LIMIT;
D O I：
10.1364/OL.38.004510
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
We implement switching laser mode coherent anti-Stokes Raman-scattering (SLAM-CARS) microscopy to enhance the spatial resolution and contrast in label-free vibrational microscopy. The method, based on the intensity difference between two images obtained with Gaussian and doughnut-shaped modes, does not depend on the specimen and relies on minimal modifications of the typical CARS setup. We demonstrate subdiffraction resolution imaging of myelin sheaths in a mouse brainstem. A lateral resolution of 0.36 lambda(p) is achieved. (C) 2013 Optical Society of America
引用
收藏
页码：4510 / 4513
页数：4
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