Resolution and contrast enhancement in coherent anti-Stokes Raman-scattering microscopy

被引：40

作者：

Gasecka, Alicja ^{[1
,2
]}

Daradich, Amy ^{[1
,2
]}

Dehez, Harold ^{[1
,2
]}

Piche, Michel ^{[2
]}

Cote, Daniel ^{[1
,2
]}

机构：

[1] Inst Univ Sante Mentale Quebec, Quebec City, PQ G1J 2G3, Canada

[2] Univ Laval, Ctr Dopt Photon & Laser, Quebec City, PQ G1V 0A6, Canada

来源：

OPTICS LETTERS | 2013年 / 38卷 / 21期

关键词：

EMISSION; BREAKING; LIMIT;

D O I：

10.1364/OL.38.004510

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We implement switching laser mode coherent anti-Stokes Raman-scattering (SLAM-CARS) microscopy to enhance the spatial resolution and contrast in label-free vibrational microscopy. The method, based on the intensity difference between two images obtained with Gaussian and doughnut-shaped modes, does not depend on the specimen and relies on minimal modifications of the typical CARS setup. We demonstrate subdiffraction resolution imaging of myelin sheaths in a mouse brainstem. A lateral resolution of 0.36 lambda(p) is achieved. (C) 2013 Optical Society of America

引用

页码：4510 / 4513

页数：4

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