Myoglobin mutant with enhanced nitrite reductase activity regulates intracellular oxidative stress in human breast cancer cells

被引：5
|
作者：
Tong, Xin-Yi [1 ]
Yang, Xin-Zhi [2 ]
Teng, Xinchen [3 ]
Gao, Shu-Qin [4 ]
Wen, Ge-Bo [4 ]
Lin, Ying-Wu [1 ,4 ]
机构：
[1] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China
[2] Univ South China, Hengyang Med Coll, Hengyang 421001, Peoples R China
[3] Soochow Univ, Coll Pharmaceut Sci, Suzhou 215123, Peoples R China
[4] Univ South China, Key Lab Prot Struct & Funct Univ Hunan Prov, Hengyang 421001, Peoples R China
来源：
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 2022年 / 730卷
基金：
中国国家自然科学基金;
关键词：
Myoglobin; Breast cancer; Nitric oxide; Nitrite reductase; Oxidative stress; OXIDE SYNTHASE; MDA-MB-468; CELLS; APOPTOSIS; THERAPY;
D O I：
10.1016/j.abb.2022.109399
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Heme proteins play vital roles in regulating the reactive oxygen/nitrogen species (ROS/RNS) levels in cells. In this study, we overexpressed human wild-type (WT) myoglobin (Mb) and its double mutant, F43H/H64A Mb with enhanced nitrite reductase (NIR) activity, in the typical representative triple-negative breast cancer cell, MDA-MB-231 cells. The results showed that the overexpression of F43H/H64A Mb increased the level of nitric oxide (NO) and the degree of oxidative stress, and then activated Akt/MAPK mediated apoptotic cascade, whereas WT Mb showed the opposite effect. This study indicates that Mb plays an important role in maintaining the balance of the cellular redox system and could thus be a valuable target for cancer therapy.
引用
收藏
页数：6
相关论文
共 50 条
  • [21] Bismuth oxide nanoparticles induce oxidative stress and apoptosis in human breast cancer cells
    Ali Alamer
    Daoud Ali
    Saud Alarifi
    Abdullah Alkahtane
    Mohammed AL-Zharani
    Mohamed M. Abdel-Daim
    Gadah Albasher
    Rafa Almeer
    Nouf K. Al-Sultan
    Abdulaziz Almalik
    Ali H Alhasan
    Christos Stournaras
    Saquib Hasnain
    Saad Alkahtani
    Environmental Science and Pollution Research, 2021, 28 : 7379 - 7389
  • [22] Antitumor Action of Amygdalin on Human Breast Cancer Cells by Selective Sensitization to Oxidative Stress
    Abboud, Muayad Mehdi
    Al Awaida, Wajdy
    Alkhateeb, Hakam Hasan
    Abu-Ayyad, Asia Numan
    NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, 2019, 71 (03): : 483 - 490
  • [23] Bismuth oxide nanoparticles induce oxidative stress and apoptosis in human breast cancer cells
    Alamer, Ali
    Ali, Daoud
    Alarifi, Saud
    Alkahtane, Abdullah
    AL-Zharani, Mohammed
    Abdel-Daim, Mohamed M.
    Albasher, Gadah
    Almeer, Rafa
    Al-Sultan, Nouf K.
    Almalik, Abdulaziz
    Alhasan, Ali H.
    Stournaras, Christos
    Hasnain, Saquib
    Alkahtani, Saad
    ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 2021, 28 (06) : 7379 - 7389
  • [24] Methylglyoxal augments intracellular oxidative stress in human aortic endothelial cells
    Miyazawa, Noriko
    Abe, Michiaki
    Souma, Tomokazu
    Tanemoto, Masayuki
    Abe, Takaaki
    Nakayama, Masaaki
    Ito, Sadayoshi
    FREE RADICAL RESEARCH, 2010, 44 (01) : 101 - 107E1
  • [25] Nitrite reductase activity of myoglobin regulates respiration and cellular viability in myocardial ischemia-reperfusion injury (vol 105, pg 10256, 2008)
    Hendgen-Cotta, Ulrike B.
    Merx, Marc W.
    Shiva, Sruti
    Schmitz, Joel
    Becher, Stefanie
    Klare, Johann P.
    Steinhoff, Heinz-Juergen
    Goedecke, Axel
    Schrader, Juer-gen
    Gladwin, Mark T.
    Kelm, Malte
    Rassaf, Tienush
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (34) : 12636 - 12636
  • [26] Intracellular uptake and inhibitory activity of aromatic fluorinated amino acids in human breast cancer cells
    Giese, Christoph
    Lepthien, Sandra
    Metzner, Linda
    Brandsch, Matthias
    Budisa, Nediljko
    Lilie, Hauke
    CHEMMEDCHEM, 2008, 3 (09) : 1449 - 1456
  • [27] Mutant TNFα negatively regulates human breast cancer stem cells from MCF7 in vitro
    Li, Yaochen
    Kong, Linghong
    Yang, Ye
    Li, Kangsheng
    CANCER BIOLOGY & THERAPY, 2007, 6 (09) : 1480 - 1489
  • [28] Molecular Analysis of BRCA1 in Human Breast Cancer Cells Under Oxidative Stress
    Gilmore, Brian L.
    Liang, Yanping
    Winton, Carly E.
    Patel, Kaya
    Karageorge, Vasilea
    Varano, A. Cameron
    Dearnaley, William
    Sheng, Zhi
    Kelly, Deborah F.
    SCIENTIFIC REPORTS, 2017, 7 : 1 - 9
  • [29] Molecular Analysis of BRCA1 in Human Breast Cancer Cells Under Oxidative Stress
    Brian L. Gilmore
    Yanping Liang
    Carly E. Winton
    Kaya Patel
    Vasilea Karageorge
    A. Cameron Varano
    William Dearnaley
    Zhi Sheng
    Deborah F. Kelly
    Scientific Reports, 7
  • [30] Effects of Selenium Yeast on Oxidative Stress, Growth Inhibition, and Apoptosis in Human Breast Cancer Cells
    Guo, Chih-Hung
    Hsia, Simon
    Shih, Min-Yi
    Hsieh, Fang-Chin
    Chen, Pei-Chung
    INTERNATIONAL JOURNAL OF MEDICAL SCIENCES, 2015, 12 (09): : 748 - 758
12345