Myoglobin mutant with enhanced nitrite reductase activity regulates intracellular oxidative stress in human breast cancer cells

被引：5
|
作者：
Tong, Xin-Yi [1 ]
Yang, Xin-Zhi [2 ]
Teng, Xinchen [3 ]
Gao, Shu-Qin [4 ]
Wen, Ge-Bo [4 ]
Lin, Ying-Wu [1 ,4 ]
机构：
[1] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China
[2] Univ South China, Hengyang Med Coll, Hengyang 421001, Peoples R China
[3] Soochow Univ, Coll Pharmaceut Sci, Suzhou 215123, Peoples R China
[4] Univ South China, Key Lab Prot Struct & Funct Univ Hunan Prov, Hengyang 421001, Peoples R China
来源：
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 2022年 / 730卷
基金：
中国国家自然科学基金;
关键词：
Myoglobin; Breast cancer; Nitric oxide; Nitrite reductase; Oxidative stress; OXIDE SYNTHASE; MDA-MB-468; CELLS; APOPTOSIS; THERAPY;
D O I：
10.1016/j.abb.2022.109399
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Heme proteins play vital roles in regulating the reactive oxygen/nitrogen species (ROS/RNS) levels in cells. In this study, we overexpressed human wild-type (WT) myoglobin (Mb) and its double mutant, F43H/H64A Mb with enhanced nitrite reductase (NIR) activity, in the typical representative triple-negative breast cancer cell, MDA-MB-231 cells. The results showed that the overexpression of F43H/H64A Mb increased the level of nitric oxide (NO) and the degree of oxidative stress, and then activated Akt/MAPK mediated apoptotic cascade, whereas WT Mb showed the opposite effect. This study indicates that Mb plays an important role in maintaining the balance of the cellular redox system and could thus be a valuable target for cancer therapy.
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页数：6
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