Cryo-EM and single particles

被引：29
|
作者：
Wang, LG [1 ]
Sigworth, FJ [1 ]
机构：
[1] Yale Univ, Sch Med, Dept Cellular & Mol Physiol, New Haven, CT 06510 USA
来源：
PHYSIOLOGY | 2006年 / 21卷
关键词：
D O I：
10.1152/physiol.00045.2005
中图分类号：
Q4 [生理学];
学科分类号：
071003 ;
摘要：
Cryoelectronmicroscopy is a method for the imaging of macromolecules in the electron microscope. It was originally developed to determine membrane protein structures from two-dimensional crystals, but more recently "single-particle" techniques have become powerful and popular. Three-dimensional reconstructions are obtained from sets of single-particle images by extensive computer processing; the methods are being applied to many macromolecular assemblies.
引用
收藏
页码：13 / 18
页数：6
相关论文
共 50 条
  • [41] Cryo-EM of Helical Polymers
    Wang, Fengbin
    Gnewou, Ordy
    Solemanifar, Armin
    Conticello, Vincent P.
    Egelman, Edward H.
    CHEMICAL REVIEWS, 2022, 122 (17) : 14055 - 14065
  • [42] Cryo-EM strikes gold
    Allison Doerr
    Nature Methods, 2015, 12 : 102 - 102
  • [43] Improving the efficiency of cryo-EM
    Nature Methods, 2017, 14 (12) : 1129 - 1129
  • [44] Cryo-Em Snapshots of the Spliceosome
    Nagai, Kiyoshi
    BIOPHYSICAL JOURNAL, 2018, 114 (03) : 366A - 366A
  • [45] The Current Revolution in Cryo-EM
    Egelman, Edward H.
    BIOPHYSICAL JOURNAL, 2016, 110 (05) : 1008 - 1012
  • [46] Graphene sandwich for cryo-EM
    Singh, Arunima
    NATURE METHODS, 2024, 21 (03) : 371 - 371
  • [47] Ribosomes and cryo-EM: a duet
    Brown, Alan
    Shao, Sichen
    CURRENT OPINION IN STRUCTURAL BIOLOGY, 2018, 52 : 1 - 7
  • [48] Cheaper cryo-EM on the horizon
    Peplow, Mark
    CHEMICAL & ENGINEERING NEWS, 2020, 98 (46) : 7 - 7
  • [49] Cryo-EM in drug discovery
    Ceska, Tom
    Chung, Chun-Wa
    Cooke, Rob
    Phillips, Chris
    Williams, Pamela A.
    BIOCHEMICAL SOCIETY TRANSACTIONS, 2019, 47 : 281 - 293
  • [50] cisTEM software for cryo-EM
    Nature Methods, 2018, 15 (5) : 314 - 314
12345