Minoxidil-induced cardiac hypertrophy in guinea pigs

被引:8
|
作者
van der Velden, J [1 ]
Borgdorff, P [1 ]
Stienen, GJM [1 ]
机构
[1] Free Univ Amsterdam, Physiol Lab, NL-1081 BT Amsterdam, Netherlands
关键词
guinea pig; cardiac hypertrophy; ATPase activity; force development; calcium sensitivity; contractile proteins; myosin heavy chains;
D O I
10.1007/s000180050332
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
To investigate whether during cardiac hypertrophy changes occur in contractile protein composition and in mechanical and energetic properties of the myocardium, contractile protein composition, isometric force and adenosine triphosphate (ATP) consumption were studied in control and hypertrophied guinea-pig hearts. Cardiac hypertrophy was induced by adding minoxidil (120 or 200 mg/l) to the drinking water. Protein analysis was performed by one-dimensional gel electrophoresis. The myosin heavy-chain (MHC) composition was determined in an enzyme-linked immunosorbent assay (ELISA). ATP consumption and force development were simultaneously measured during isometric contraction in chemically skinned trabeculae. Histochemical analysis of cross-sectional. area of cardiomyocytes and interstitial space was performed on the left ventricular tissue of 200 mg/l minoxidil-treated and control guinea pigs. Minoxidil treatment (120 and 200 mg/l) significantly increased left ventricular dry weight normalized for body weight by 19 +/- 4 and 24 +/- 4%, respectively. No significant differences were found in the cellular cross-sectional area, while interstitial space was slightly decreased in minoxidil-treated hearts. In left ventricular trabeculae of 200 mg/l minoxidil-treated guinea pigs, ATPase activity was slightly less than in those of control guinea pigs, whereas force did not differ significantly. Calcium sensitivity of force and ATPase activity were not affected by minoxidil treatment. Gel electrophoresis revealed no difference in contractile protein composition, but a tendency towards a lower amount of alpha-MHC in the minoxidil-treated hearts was found in ELISA.
引用
收藏
页码:788 / 798
页数:11
相关论文
共 50 条
  • [21] MINOXIDIL-INDUCED PERICARDIAL EFFUSION: A RARE SIDE EFFECT
    Mohanakrishnan, B.
    Daines, B.
    Hasan, M.
    Saadaldin, M.
    JOURNAL OF INVESTIGATIVE MEDICINE, 2022, 70 (02) : 635 - 636
  • [22] STRUCTURAL AND FUNCTIONAL CORRELATES OF MINOXIDIL INDUCED CARDIAC-HYPERTROPHY
    MORAVEC, CS
    RUHE, T
    CIFANI, JR
    MILOVANOVIC, M
    FASEB JOURNAL, 1991, 5 (05): : A1435 - A1435
  • [23] CARDIAC HYPERTROPHY AND HEART-FAILURE IN GUINEA-PIGS
    CALVELEY, SB
    PATHOLOGY, 1973, 5 (01) : 79 - 79
  • [24] Minoxidil-induced hypertrichosis in an 8-month-old infant
    Gomaa, Ahmed S.
    Badran, Aya
    Kamaleldeen, Eman B.
    INTERNATIONAL JOURNAL OF DERMATOLOGY, 2020, 59 (01) : E14 - E15
  • [25] INFLUENCE OF CHRONIC DIURETIC THERAPY ON MINOXIDIL-INDUCED NECROSIS IN DOGS
    HERMAN, E
    BALAZS, T
    EARL, F
    KROP, S
    TOXICOLOGY AND APPLIED PHARMACOLOGY, 1975, 33 (01) : 198 - 199
  • [26] MINOXIDIL-INDUCED SEVERE REVERSIBLE NON-ISCHEMIC CARDIOMYOPATHY
    Stephen, Reejis
    Toledo, Clarisse
    Pohl, Marc A.
    AMERICAN JOURNAL OF KIDNEY DISEASES, 2015, 65 (04) : A81 - A81
  • [27] Atypical Case of Minoxidil-Induced Generalized Anasarca and Pleuropericardial Effusion
    Gbadamosi, Wahab A.
    Melvin, Jacqueline
    Lopez, Marvin
    CUREUS JOURNAL OF MEDICAL SCIENCE, 2021, 13 (06)
  • [28] INTERACTION OF RESERPINE AND FUROSEMIDE ON MINOXIDIL-INDUCED MYOCARDIAL NECROSIS IN DOGS
    HERMAN, E
    BALAZS, T
    YOUNG, R
    EARL, F
    KROP, S
    PHARMACOLOGIST, 1975, 17 (02): : 204 - 204
  • [29] Minoxidil-induced alteration of corneal topography after radial keratotomy
    Goins, KM
    Karp, KO
    Gabrielian, K
    JOURNAL OF CATARACT AND REFRACTIVE SURGERY, 1998, 24 (05): : 612 - 618
  • [30] Minoxidil-induced hypertrichosis in a 4-year-old child
    Verma, Shyam B.
    INDIAN JOURNAL OF DERMATOLOGY VENEREOLOGY & LEPROLOGY, 2016, 82 (03): : 304 - 305