PHGDH is an independent prognosis marker and contributes cell proliferation, migration and invasion in human pancreatic cancer

被引：65
|
作者：
Song, Zhiwang [1 ]
Feng, Chan [1 ]
Lu, Yonglin [1 ]
Lin, Yun [1 ]
Dong, Chunyan [1 ]
机构：
[1] Tongji Univ, Sch Med, Shanghai East Hosp, Dept Oncol, 150 Jimo Rd, Shanghai 200120, Peoples R China
来源：
GENE | 2018年 / 642卷
关键词：
Pancreatic cancer; Phosphoglycerate dehydrogenase (PHGDH); Proliferation; Prognosis; PHOSPHOGLYCERATE DEHYDROGENASE; HIGH EXPRESSION; POOR-PROGNOSIS; METASTASIS; STATISTICS; METABOLISM;
D O I：
10.1016/j.gene.2017.11.014
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Purpose: To investigate the expression, clinical significance, biological function, and the potential mechanism of PHGDH in pancreatic cancer. Methods: The expression of PHGDH in human pancreatic cancer tissues and corresponding adjacent normal tissues were analyzed through immunohistochemistry staining. Simultaneously, the association between the PHGDH expression and the clinicopathological parameters and OS and DFS was evaluated. Human pancreatic cancer cell line BxPC-3 and SW1990 were selected to investigate the effect of PHGDH knockdown on cell proliferation, migration, and invasion. In addition, we performed western blot to assess the expression of cyclin and cyclin D1, MMP-2, and MMP-9 protein. Results: Our results suggested that the expression of PHGDH is increased in pancreatic cancer compared with adjacent normal tissues and the increased expression of PHGDH is associated with tumor size, lymph node metastasis, and TNM state of pancreatic cancer patients. Moreover, the expression of PHGDH is an independent prognostic indicator for pancreatic cancer patients. In addition, we found that knockdown of PHGDH in pancreatic cancer cells inhibits the cell proliferation, migration, and invasion abilities by down-regulating the expression of cyclin B1, and cyclin Dl, MMP-2, and MMP-9. Conclusions: Our data indicated that the expression of PHGDH is increased in pancreatic cancer and is an independent molecular prognostic factor for pancreatic cancer patients. In addition, PHGDH controls cell proliferation, migration and invasion abilities. Therefore, PHGDH could serve as an important prognostic indicator and therapeutic target for pancreatic cancer.
引用
收藏
页码：43 / 50
页数：8
相关论文
共 50 条
  • [31] TRIM29 promotes gastric cancer cell proliferation, migration, invasion and predicts the prognosis of patients
    Huang, Jinxi
    Zhou, Yi
    Wang, Chenghu
    Yuan, Weiwei
    Zhang, Zhandong
    Chen, Beibei
    Zhang, Xiefu
    INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE, 2018, 11 (08): : 7936 - 7944
  • [32] TRIP13 is a predictor for poor prognosis and regulates cell proliferation, migration and invasion in prostate cancer
    Dong, Liming
    Ding, Honglin
    Li, Yanpei
    Xue, Dongwei
    Li, Zhi
    Liu, Yili
    Zhang, Teng
    Zhou, Jian
    Wang, Ping
    INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2019, 121 : 200 - 206
  • [33] MicroRNA-4317 predicts the prognosis of breast cancer and inhibits tumor cell proliferation, migration, and invasion
    Yuwei Sheng
    Rong Hu
    Yi Zhang
    Wenjie Luo
    Clinical and Experimental Medicine, 2020, 20 : 417 - 425
  • [34] Necroptosis at Invasion Front of Pancreatic Cancer Can Promote Cancer Cell Migration and Invasion
    Ando, Y.
    Ohuchida, K.
    Yonenaga, A.
    Sagara, A.
    Kibe, S.
    Takesue, S.
    Nakayama, M.
    Shindo, K.
    Moriyama, T.
    Nakata, K.
    Miyasaka, Y.
    Ohtsuka, T.
    Mizumoto, K.
    Nakamura, M.
    PANCREAS, 2018, 47 (10) : 1371 - 1371
  • [35] AEBP1 Contributes to Breast Cancer Progression by Facilitating Cell Proliferation, Migration, Invasion, and Blocking Apoptosis
    Li, Jin
    Ruan, Yanyun
    Zheng, Chenhui
    Pan, Yue
    Lin, Bangyi
    Chen, Qi
    Zheng, Zhibao
    DISCOVERY MEDICINE, 2023, 35 (174) : 45 - 56
  • [36] CD133 POSITIVE CELLS IN PANCREATIC CANCER POSSESS INCREASED CELL PROLIFERATION, MIGRATION AND INVASION
    Moriyama, T.
    Ohuchida, K.
    Mizumoto, K.
    Lin, C.
    Sato, N.
    Tanaka, M.
    PANCREAS, 2008, 37 (04) : 485 - 485
  • [37] Ethyl Acetate Extract of Maple Syrup Inhibited Cell Proliferation, Migration, and Invasion in Pancreatic Cancer Cells
    Yamamoto, T.
    Kuragaki, M.
    Inoue, R.
    Mitamura, K.
    Taga, A.
    PANCREAS, 2018, 47 (10) : 1434 - 1434
  • [38] Expression of KLF9 in pancreatic cancer and its effects on the invasion, migration, apoptosis, cell cycle distribution, and proliferation of pancreatic cancer cell lines
    Zhong, Zhiwei
    Zhou, Fan
    Wang, Dong
    Wu, Mingming
    Zhou, Weimin
    Zou, Yeqing
    Li, Jie
    Wu, Linquan
    Yin, Xiangbao
    ONCOLOGY REPORTS, 2018, 40 (06) : 3852 - 3860
  • [39] Impact of protamine I on colon cancer proliferation, invasion, migration, diagnosis and prognosis
    Chen, Zhi
    Shi, Chunyu
    Gao, Shuohui
    Song, Defeng
    Feng, Ye
    BIOLOGICAL CHEMISTRY, 2018, 399 (03) : 265 - 275
  • [40] High expression of HMBOX1 contributes to poor prognosis of gastric cancer by promoting cell proliferation and migration
    Diao, Nannan
    Li, Yuzheng
    Yang, Jinling
    Jin, Chengjuan
    Meng, Xiaohui
    Jiao, Wenlin
    Feng, Jinbo
    Liu, Zhenping
    Lu, Nan
    BIOMEDICINE & PHARMACOTHERAPY, 2019, 115
12345