Universal and reusable solide support thanks to a H-phosphonate diester linkage for the synthesis of single or multiple oligonucleotides

被引：0

作者：

Ferreira, F ^{[1
]}

Meyer, A ^{[1
]}

Vasseur, JJ ^{[1
]}

Morvan, F ^{[1
]}

机构：

[1] Univ Montpellier 2, Lab Chim Organ Biomol Synth, F-34095 Montpellier, France

来源：

Chemistry of Nucleic Acid Components | 2005年 / 7卷

关键词：

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An universal and reusable solid support exhibiting a hydroxyl function was prepared. Thanks to a first H-phosphonate diester linkage which was kept non-oxidized all along the elongation, 3'-hydroxyl oligonucleotides were released by a transesterification mechanism. The transesterification was performed within few minutes with either ethanolamine or K2CO3/methanol and the solid support was recycled by a simple washing and drying.

引用

页码：149 / 152

页数：4