The Distribution of Synaptotagmin II in RBL-2H3 and Its Regulation on Exocytosis of Lysosomes in RBL-2H3

被引：0
|
作者：
Zhang, Jicheng [1 ]
Wu, Jianmin [1 ]
Pan, Shixiu [1 ]
Lv, Wenli [2 ]
机构：
[1] Huazhong Univ Sci & Technol, Tongji Med Coll, Dept Lab Med, Union Hosp, Wuhan 430022, Peoples R China
[2] Wuhan Inst Biol Prod, Wuhan 430060, Peoples R China
来源：
CELLULAR & MOLECULAR IMMUNOLOGY | 2005年 / 2卷 / 03期
基金：
中国国家自然科学基金;
关键词：
calcium binding protein; mast cell; gene expression; lysosome; exocytosis;
D O I：
暂无
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Synaptotagmin (Syt) constitutes a family of membrane-trafficking proteins, so far nearly 20 Syts have been discovered. Extensive work showed that synatotagmins were a potential Ca2+ sensor for regulated exocytosis. This study was to investigate the expression and location of synaptotagmin II (Syt2) in RBL-2H3 (RBL) and its role in regulating exocytosis of RBL. The expression of Syt2 in RBL was confirmed by Western blot. The recombinant expression vector pEGFP-N1-Syt2 was constructed and transfected into RBL by electroporation, the stable transfectant RBL-Syt2-S expressing fusion protein Syt2-EGFP were obtained and Syt2 was highly concentrated at plasma membrane with little detected in cytoplasm. To analyze the role of Syt2 during exocytosis of RBL, the release of cathepsin D was assayed by immunoblotting. Compared with control, the release of cathepsin D by RBL-Syt2-S was markedly decreased. The results indicated that Syt2 played a negative regulation in exocytosis of lysosomes in RBL.
引用
收藏
页码：205 / 209
页数：5
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