Correlation between IL-1 β, IL-1Ra gene polymorphism and occurrence of polycystic ovary syndrome infertility

Objective: To explore the relationship between IL-1 beta, IL-1Ra gene polymorphism and the occurrence of polycystic ovary syndrome (PCOS) infertility. Methods: A total of 59 PCOS infertility cases visiting the reproductive center of our hospital from Mar. 2010 to Mar. 2012 and 56 healthy women were selected. ELISA method was used for the detection of IL-1 beta, IL-1Ra levels, and the levels of serum supersensitivity C reaction protein US-CRP), insulin (FINS), follicule-stimulating hormone (FSH) and fasting blood glucose (FRG) were detected. PCR analysis technology was adopted to detect the gene polymorphism of the 511 site of IL-1 beta and the second intronc of IL-1Ra. Results: The levels of IL-1 beta, IL-1Ra, US-CRP, FINS and FBG in blood scrum of patients in PCOS group were significantly higher than those in control group (P<0.05 or P<0.01). The level of FSH in PCOS group was significantly lower than that in control group (P<0.05). The genotypic frequency of T/T, the 511 site of IL-1 (3 in PCOS group was 42.37%, significantly higher than 12.50% in control group (P<0.01). The frequency of T allele was also significantly higher than that in control group (P<0.01). The genotypic frequency of I/V, the second introne of IL-1Ra in PCOS group was 20.34%, signiciantly higher than 3.57% in control group (P<0.05). The frequency of V allele in PCOS group was significantly higher than that in control group (P<0.05). Conclusions: T allele of the 511 site of IL-1 beta gene and V allele of the second introne of IL-1Ra gene might be the genetic basis of the rising of IL-1 beta, IL-1Ra and US-CRP levels in blood serum of PCOS patients, and are associated with the infertility occurrence of PCOS patients.