Validation of HPLC Method for Determination of trans,trans-Muconic Acid as Bio-marker of Benzene Exposure

被引：0
|
作者：
Bajcsova, Ivica [1 ]
Legath, L'ubomir [2 ]
Gondova, Tatana [3 ]
Vargova, Zuzana [4 ]
机构：
[1] Univ Nemocnica L Pasteura Kosice, Klin Pracovneho Lekarstva & Klin Toxikol, Kosice 04001, Slovakia
[2] Safarik Univ, Klin Pracovneho Lekarstva & Klin Toxikol, Lekarska Fak, Kosice 04001, Slovakia
[3] Safarik Univ, Prirodovedecka Fak, Katedra Analytickej Chem, Kosice 04001, Slovakia
[4] Safarik Univ, Prirodovedecka Fak, Katedra Anorganickej Chem, Kosice 04001, Slovakia
来源：
CHEMICKE LISTY | 2012年 / 106卷 / 04期
关键词：
TRANS-MUCONIC ACID; URINARY TRANS; WORKERS; BIOMARKERS;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Determination of phenol in urine is a widely used method. However, it may provide false positive results due to possible presence of aromatic amino acids, which can also produce phenol in urine. Trans,trans-muconic acid (MA) is a non-phenolic metabolite of benzene which appears in urine. The present review deals with determination of MA by RP-HPLC with UV detection. MA was isolated from urine by solid phase extraction (SPE). The mean recovery was 96-103 %. The detection and quantification limits were 0.057 mg l(-1) and 0.189 mg l(-1), respectively. This method can be used to determine, control and monitor the level of MA in urine of workers, who are overexposed to benzene. The obtained data were compared with those of the non-exposed control group. The results suggest that MA could be one of the validated benzene bio-markers in risk assessment.
引用
收藏
页码：293 / 298
页数：6
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