Target DNA induced switches of DNA polymerase activity

被引：33
|
作者：
Park, Ki Soo [1 ]
Lee, Chang Yeol [1 ]
Park, Hyun Gyu [1 ]
机构：
[1] Korea Adv Inst Sci & Technol, Dept Chem & Biomol Engn, Program BK21, Taejon 305701, South Korea
来源：
CHEMICAL COMMUNICATIONS | 2015年 / 51卷 / 49期
基金：
新加坡国家研究基金会;
关键词：
APTAMERIC ENZYME SUBUNIT; GRAPHENE OXIDE; FLUORESCENCE DETECTION; MOLECULAR BEACONS; PROBES; HYBRIDIZATION; PCR; POLYMERIZATION; PLATFORM; BINDING;
D O I：
10.1039/c5cc02060c
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A novel concept that target DNA can induce switching of DNA polymerase activity is devised. The method relies on the finding that a DNA aptamer can undergo conformational change upon hybridization with a complementary target DNA, which leads to activation or inactivation of DNA polymerase. This strategy is utilized to identify the presence of target DNA with high levels of sensitivity and selectivity.
引用
收藏
页码：9942 / 9945
页数：4
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