Dark-field third-harmonic imaging

被引：12
|
作者：
Doronina-Amitonova, L. V. [1 ,2 ,3 ]
Lanin, A. A. [1 ,3 ]
Fedotov, I. V. [1 ,2 ,3 ]
Ivashkina, O. I. [3 ,4 ]
Zots, M. A. [3 ,4 ]
Fedotov, A. B. [1 ,2 ,3 ]
Anokhin, K. V. [3 ,4 ]
Zheltikov, A. M. [1 ,2 ,3 ,5 ,6 ]
机构：
[1] Moscow MV Lomonosov State Univ, Dept Phys, Ctr Int Laser, Moscow 119992, Russia
[2] Russian Quantum Ctr, Skolkovo 1430125, Moscow Region, Russia
[3] Natl Res Ctr, Kurchatov Inst, Lab Neurophoton, Moscow 123098, Russia
[4] Russian Acad Med Sci, PK Anokhin Inst Normal Physiol, Moscow, Russia
[5] Texas A&M Univ, Dept Phys & Astron, College Stn, TX 77843 USA
[6] Russian Acad Sci, Ctr Photochem, Moscow 117421, Russia
来源：
APPLIED PHYSICS LETTERS | 2013年 / 103卷 / 09期
基金：
俄罗斯基础研究基金会;
关键词：
MICROSCOPY; TISSUES; CELLS;
D O I：
10.1063/1.4818973
中图分类号：
O59 [应用物理学];
学科分类号：
摘要：
Coherent cancellation of third-harmonic generation (THG) in a tightly focused laser beam is shown to enable a label-free imaging of individual neurons in representative brain tissues. The intrinsic coherence of third-harmonic buildup and cancellation combined with the nonlinear nature of the process enhances the locality of the dark signal in THG, translating into a remarkable sharpness of dark-field THG images. Unique advantages of this technique for high-contrast subcellular-resolution neuroimaging are demonstrated by comparing THG images of hippocampus and somatosensory cortex in a mouse brain with images visualizing fluorescent protein biomarkers. (C) 2013 AIP Publishing LLC.
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页数：4
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