COMPARATIVE STUDY ON PHYTOCHEMICAL ANALYSIS AND ANTIBACTERIAL ACTIVITIES OF RAISINS (VITIS VINIFERA L.)

The study was investigated to evaluate the phytochemical analysis and Antibacterial activities of Vitis vinifera. Vitis vinifera belongs to the family Vitaceae and seen in tropical, subtropical and warm temperate regions. The quantitative phytochemical analysis of Vitis vinifera exhibited the presence of secondary metabolites such as terpenoids, steroids, phenolic compounds, flavonoids, glycosides and saponins. Antibacterial activity was performed against Gram Positive (Bacillus subtilis, Micrococcus luteus, Staphylococcus aureus) and Gram Negative Microbes (Proteus vulgaris and Shigella flexneri) using Agar-well diffusion methods. It was found that Bacillus subtilis showed highest sensitivity of 13mm and 20mm at 625 mu g of concentrations in both black and green raisins respectively among Gram-positive bacteria. In Gram-negative Shigella flexneri showed 12mm and 20mm zone of inhibition at 625 mu g of concentration in both black and green raisins respectively where as Proteus vulgaris showed no activity in green raisin. Thus, from the study it can be concluded that Vitis vinifera produce various bioactive compounds and have an upstanding Antibacterial activity.