SPR bio-sensor using white light source and OSA

In this study, we fabricated wavelength type SPR (surface plasmon resonance) sensor to measure microscopic refractive index change in wide band by real time. We measured SPR phenomena according to salmonella antigen-antibody reaction and penicillinase - penicillin reaction to apply in SPR immunosensor. Our experiments used a SAM (self assembled monolayer) method to immobilize Salmonella antibody and penicillinase on each sensor chips. Also, Salmonella antigen and penicillin poured within each antibody immobilized sensor chambers. Concentrations of Salmonella antibody and antigen are 50 mu m/ml and 5 x 10(7) cfu/ml. Concentration of penicillinase and penicillin are 0.5 mg/ml and 10 mM. The shifts of resonance wavelength for each experiment were measured using OSA for 30 minutes. We could know that salmonella antigen-antibody reactions were saturated after 10 minutes and penicillinase - penicillin reactions were saturated after 15 minutes. The shift of resonance wavelength was varied about 0.26 nm/min for Salmonella antigen-antibody reaction and 0.8 nm/min for penicillinase - penicillin reaction.