Development of a Monoclonal Antibody-Based Sandwich ELISA for Peanut Allergen Ara h 1 in Food

被引：34

作者：

Peng, Juan ^{[1
]}

Song, Shanshan ^{[1
]}

Xu, Liguang ^{[1
]}

Ma, Wei ^{[1
]}

Liu, Liqiang ^{[1
]}

Kuang, Hua ^{[1
]}

Xu, Chuanlai ^{[1
]}

机构：

[1] Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China

来源：

INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH | 2013年 / 10卷 / 07期

基金：

中国国家自然科学基金;

关键词：

peanut allergen; Ara h 1; monoclonal antibody; sandwich ELISA; ANAPHYLACTIC REACTIONS; DOUBLE-BLIND; NUT ALLERGY; ARA-H-1;

D O I：

10.3390/ijerph10072897

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

We have established a highly sensitive sandwich enzyme-linked immunosorbent assay (ELISA) based on two monoclonal antibodies (mAb) to measure the content of the major peanut allergen Ara h 1 in foods. Two mAbs were selected out of 12 murine hybridoma cells secreting Ara h 1-specific antibody. Using mAb 6 as the capture antibody and HRP-labelled mAb 4 as the detection antibody, the limit of detection (LOD) the assay was 0.34 ng/mL. Cross-reaction analysis showed that this method was strongly specific and had no cross-reactions with Ara h 2, pea protein or soy protein. Sample analysis showed that this ELISA was a useful tool to monitor peanut allergens in food products by measuring Ara h 1 content.

引用

页码：2897 / 2905

页数：9

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