Development of a Monoclonal Antibody-Based Sandwich ELISA for Peanut Allergen Ara h 1 in Food

被引：34
|
作者：
Peng, Juan [1 ]
Song, Shanshan [1 ]
Xu, Liguang [1 ]
Ma, Wei [1 ]
Liu, Liqiang [1 ]
Kuang, Hua [1 ]
Xu, Chuanlai [1 ]
机构：
[1] Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China
来源：
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH | 2013年 / 10卷 / 07期
基金：
中国国家自然科学基金;
关键词：
peanut allergen; Ara h 1; monoclonal antibody; sandwich ELISA; ANAPHYLACTIC REACTIONS; DOUBLE-BLIND; NUT ALLERGY; ARA-H-1;
D O I：
10.3390/ijerph10072897
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
We have established a highly sensitive sandwich enzyme-linked immunosorbent assay (ELISA) based on two monoclonal antibodies (mAb) to measure the content of the major peanut allergen Ara h 1 in foods. Two mAbs were selected out of 12 murine hybridoma cells secreting Ara h 1-specific antibody. Using mAb 6 as the capture antibody and HRP-labelled mAb 4 as the detection antibody, the limit of detection (LOD) the assay was 0.34 ng/mL. Cross-reaction analysis showed that this method was strongly specific and had no cross-reactions with Ara h 2, pea protein or soy protein. Sample analysis showed that this ELISA was a useful tool to monitor peanut allergens in food products by measuring Ara h 1 content.
引用
收藏
页码：2897 / 2905
页数：9
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