Transcriptome and selected metabolite analyses reveal multiple points of ethylene control during tomato fruit development

被引:409
|
作者
Alba, R
Payton, P
Fei, ZJ
McQuinn, R
Debbie, P
Martin, GB
Tanksley, SD
Giovannoni, JJ
机构
[1] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA
[2] ARS, USDA, Plant Soil & Nutr Lab, Ithaca, NY 14853 USA
[3] Cornell Univ, Dept Plant Pathol, Ithaca, NY 14853 USA
[4] Cornell Univ, Dept Plant Breeding, Ithaca, NY 14853 USA
来源
PLANT CELL | 2005年 / 17卷 / 11期
关键词
D O I
10.1105/tpc.105.036053
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Transcriptome profiling via cDNA microarray analysis identified 869 genes that are differentially expressed in developing tomato (Solanum lycopersicum) pericarp. Parallel phenotypic and targeted metabolite comparisons were employed to inform the expression analysis. Transcript accumulation in tomato fruit was observed to be extensively coordinated and often completely dependent on ethylene. Mutation of an ethylene receptor (Never-ripe [Nr]), which reduces ethylene sensitivity and inhibits ripening, alters the expression of 37% of these 869 genes. Nr also influences fruit morphology, seed number, ascorbate accumulation, carotenoid biosynthesis, ethylene evolution, and the expression of many genes during fruit maturation, indicating that ethylene governs multiple aspects of development both prior to and during fruit ripening in tomato. Of the 869 genes identified, 628 share homology (E-value <= 1 x 10(-10)) with known gene products or known protein domains. Of these 628 loci, 72 share homology with previously described signal transduction or transcription factors, suggesting complex regulatory control. These results demonstrate multiple points of ethylene regulatory control during tomato fruit development and provide new insights into the molecular basis of ethylene-mediated ripening.
引用
收藏
页码:2954 / 2965
页数:12
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