Immunohistochemical analysis of β3 integrin (CD61):: expression in pig tissues and human tumors

被引:0
|
作者
Moreno, A
Lucena, C
López, A
Garrido, JJ
de la Lastra, JMP
Llanes, D
机构
[1] Univ Cordoba, Spanish Res Council, Dept Genet, Cordoba 14071, Spain
[2] Univ Cordoba, Fac Med, Dept Cell Biol, Cordoba 14071, Spain
[3] Univ Cordoba, Fac Med, Dept Med Surg, Cordoba 14071, Spain
关键词
beta(3) integrin; immunohistochemistry; pig tissues; human tumors; monoclonal antibody;
D O I
暂无
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
CD61 is a membrane glycoprotein that associates with CD41 (aalphaIIb) to form the heterodimeric complex gpIIb/IIIa (CD41/CD61), predominantly expressed in platelets and megakariocytes. CD61 or beta(3) integrin also associates with alphav (CD51) to form the vitronectin receptor, which is expressed in many tissues. We have used a monoclonal antibody against the porcine gpIIIa or CD61 (JM2E5) to study the distribution of this molecule in different normal pig tissues. As in humans, CD61 was broadly expressed in all tissues examined. In the kidney, strong expression of CD61 was observed in epithelial cells from renal tubules. In the testis, CD61 expression was detected in the Leydig cells. However, in liver, CD61 was weak or not detected. Many integrins are particularly involved in tumogenicity and in tumor progression mediating cell-cell interaction. Immunofluorescence experiments using cultured human tumor HeLa cells showed nuclear and cytoplasmic staining of mAb JM2E5. Immunohistochemical analysis of human tumor sections from several organs showed a heterogeneus distribution in metastatic cases from colon and breast carcinoma. However, no staining was found in metastasis from melanoma.
引用
收藏
页码:347 / 352
页数:6
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