Biodegradation of acetochlor by a newly isolated Achromobacter sp strain D-12

被引:14
|
作者
Xu, Chao [1 ]
Ding, Jinghui [1 ]
Qiu, Jiguo [2 ]
Ma, Yun [1 ]
机构
[1] Zhejiang Univ Technol, Res Ctr Environm Sci, Hangzhou, Zhejiang, Peoples R China
[2] Zhejiang Univ, Inst Environm Sci, Hangzhou 310003, Zhejiang, Peoples R China
基金
中国国家自然科学基金;
关键词
Acetochlor; biodegradation; isolation; Achromobacter sp; CHLOROACETANILIDE HERBICIDES; ISOMER SELECTIVITY; AQUATIC TOXICITY; GROUND-WATER; DEGRADATION; SOILS; METABOLITES; EXPRESSION; POLLUTANTS; BUTACHLOR;
D O I
10.1080/03601234.2013.816601
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
A highly effective acetochlor-degrading bacterial strain (D-12) was isolated from the soil of a pesticide factory. The strain was identified as Achromobacter sp. based on its 16S rRNA gene sequence. The strain D-12 optimally degrades acetochlor at a pH of 7.0 and a temperature of 30 degrees C in a mineral salts medium (MSM). Approximately 95% of acetochlor was degraded by the stain treated at a concentration of 10mg L-1 after 5days of incubation. A chiral high performance liquid chromatography (HPLC) system was used to study the enantioselectivity during the process. However, no obvious enantioselective biodegradation was observed. The primary biodegradation acetochlor products were identified by high-performance liquid chromatography-mass spectroscopy (HPLC-MS) and gas chromatography-mass spectrometry (GC-MS). The results indicated that the strain D-12 could be applied in the bioremediation of an acetochlor-polluted environment.
引用
收藏
页码:960 / 966
页数:7
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