Quantification of IncP-1 Plasmid Prevalence in Environmental Samples

被引:41
|
作者
Jechalke, Sven [1 ]
Dealtry, Simone [1 ]
Smalla, Kornelia [1 ]
Heuer, Holger [1 ]
机构
[1] Julius Kuhn Inst, Fed Res Ctr Cultivated Plants JKI, Dept Epidemiol & Pathogen Diagnost, Braunschweig, Germany
关键词
HOST-RANGE PLASMIDS; RESISTANCE GENES; PROMISCUOUS PLASMID; BACTERIA; POPULATIONS; TRANSPOSON; EVOLUTION; BACKBONE; SEQUENCE; DIVERSE;
D O I
10.1128/AEM.03728-12
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
To study the role of broad-host-range IncP-1 plasmids in bacterial adaptability to irregular environmental challenges, a quantitative real-time PCR assay was developed that specifically detects the korB gene, which is conserved in all IncP-1 plasmids, in environmental samples. IncP-1 plasmid dynamics in a biopurification system for pesticide wastes were analyzed.
引用
收藏
页码:1410 / 1413
页数:4
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