Quantification of IncP-1 Plasmid Prevalence in Environmental Samples

被引:41
|
作者
Jechalke, Sven [1 ]
Dealtry, Simone [1 ]
Smalla, Kornelia [1 ]
Heuer, Holger [1 ]
机构
[1] Julius Kuhn Inst, Fed Res Ctr Cultivated Plants JKI, Dept Epidemiol & Pathogen Diagnost, Braunschweig, Germany
关键词
HOST-RANGE PLASMIDS; RESISTANCE GENES; PROMISCUOUS PLASMID; BACTERIA; POPULATIONS; TRANSPOSON; EVOLUTION; BACKBONE; SEQUENCE; DIVERSE;
D O I
10.1128/AEM.03728-12
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
To study the role of broad-host-range IncP-1 plasmids in bacterial adaptability to irregular environmental challenges, a quantitative real-time PCR assay was developed that specifically detects the korB gene, which is conserved in all IncP-1 plasmids, in environmental samples. IncP-1 plasmid dynamics in a biopurification system for pesticide wastes were analyzed.
引用
收藏
页码:1410 / 1413
页数:4
相关论文
共 50 条
  • [1] REPLICATION MUTANTS OF THE INCP-1 PLASMID RP1
    WATSON, J
    EXPERIENTIA, 1980, 36 (12): : 1451 - 1451
  • [2] INCOMPATIBILITY PROPERTIES OF THE INCP-1 PLASMID PRK248
    MEYER, R
    TEXAS JOURNAL OF SCIENCE, 1979, 31 (05): : 110 - 111
  • [3] Host range diversification within the IncP-1 plasmid group
    Yano, Hirokazu
    Rogers, Linda M.
    Knox, Molly G.
    Heuer, Holger
    Smalla, Kornelia
    Brown, Celeste J.
    Top, Eva M.
    MICROBIOLOGY-SGM, 2013, 159 : 2303 - 2315
  • [4] ROLE OF INCP-1 PLASMID PRIMASE IN CONJUGATION BETWEEN PSEUDOMONAS SPECIES
    NASH, J
    KRISHNAPILLAI, V
    FEMS MICROBIOLOGY LETTERS, 1988, 49 (02) : 257 - 260
  • [5] Cultivation-Independent Screening Revealed Hot Spots of IncP-1, IncP-7 and IncP-9 Plasmid Occurrence in Different Environmental Habitats
    Dealtry, Simone
    Ding, Guo-Chun
    Weichelt, Viola
    Dunon, Vincent
    Schlueter, Andreas
    Carla Martini, Maria
    Florencia Del Papa, Maria
    Lagares, Antonio
    Amos, Gregory Charles Auton
    Wellington, Elizabeth Margaret Helen
    Gaze, William Hugo
    Sipkema, Detmer
    Sjoling, Sara
    Springael, Dirk
    Heuer, Holger
    van Elsas, Jan Dirk
    Thomas, Christopher
    Smalla, Kornelia
    PLOS ONE, 2014, 9 (02):
  • [6] Transposition of Tn21 to a cryptic IncP-1β plasmid pBPI36 helps to explain the structural similarity of IncP-1β plasmids.
    Sota, Masashiro
    Top, Eva M.
    PLASMID, 2007, 57 (02) : 230 - 230
  • [7] Iteron control of oriV function in IncP-1 plasmid RK2
    Maurya, Anand P.
    Lazdins, Alessandro
    Wilson, Helen
    Lloyd, Georgina S.
    Stephens, Elton R.
    Haines, Anthony S.
    Thomas, Christopher M.
    PLASMID, 2023, 126
  • [8] Regulation of IncP-1 plasmid, RK2, backbone functions.
    不详
    PLASMID, 2005, 53 (01) : 86 - 86
  • [9] Distribution of regulatory proteins on the genome of IncP-1 plasmid RK2.
    不详
    PLASMID, 2005, 53 (01) : 85 - 86
  • [10] Distribution of the partitioning protein KorB on the genome of IncP-1 plasmid RK2
    Chiu, Chung-Min
    Manzoor, Susan E.
    Batt, Sarah M.
    Muntaha, Sidra Tul
    Bingle, Lewis E. H.
    Thomas, Christopher M.
    PLASMID, 2008, 59 (03) : 163 - 175