A Hyaluronan-Based Injectable Hydrogel Improves the Survival and Integration of Stem Cell Progeny following Transplantation

被引：183
|
作者：
Ballios, Brian G. [1 ]
Cooke, Michael J. [2 ]
Donaldson, Laura [3 ]
Coles, Brenda L. K. [4 ]
Morshead, Cindi M. [5 ,6 ]
van der Kooy, Derek [4 ,6 ]
Shoichet, Molly S. [2 ,6 ]
机构：
[1] Univ Toronto, Fac Med, Toronto, ON M5S 1A8, Canada
[2] Univ Toronto, Dept Chem Engn & Appl Chem, Toronto, ON M5S 3E5, Canada
[3] McMaster Univ, Div Ophthalmol, Dept Surg, Fac Hlth Sci, Hamilton, ON L8G 4X3, Canada
[4] Univ Toronto, Dept Mol Genet, Toronto, ON M5S 1A8, Canada
[5] Univ Toronto, Dept Surg, Toronto, ON M5S 1A8, Canada
[6] Univ Toronto, Donnelly Ctr, Toronto, ON M5S 3E1, Canada
来源：
STEM CELL REPORTS | 2015年 / 4卷 / 06期
关键词：
INJURED SPINAL-CORD; PHOTORECEPTOR PRECURSORS; RETINAL REPAIR; MOUSE RETINA; RAT MODEL; DELIVERY; CD44; MICE; STROKE; DIFFERENTIATION;
D O I：
10.1016/j.stemcr.2015.04.008
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
The utility of stem cells and their progeny in adult transplantation models has been limited by poor survival and integration. We designed an injectable and bioresorbable hydrogel blend of hyaluronan and methylcellulose (HAMC) and tested it with two cell types in two animal models, thereby gaining an understanding of its general applicability for enhanced cell distribution, survival, integration, and functional repair relative to conventional cell delivery in saline. HAMC improves cell survival and integration of retinal stem cell (RSC)-derived rods in the retina. The pro-survival mechanism of HAMC is ascribed to the interaction of the CD44 receptor with HA. Transient disruption of the retinal outer limiting membrane, combined with HAMC delivery, results in significantly improved rod survival and visual function. HAMC also improves the distribution, viability, and functional repair of neural stem and progenitor cells (NSCs). The HAMC delivery system improves cell transplantation efficacy in two CNS models, suggesting broad applicability.
引用
收藏
页码：1031 / 1045
页数：15
相关论文
共 50 条
  • [31] Hyaluronan Mixed Esters of Butyric and Retinoic Acid Affording Myocardial Survival and Repair without Stem Cell Transplantation
    Lionetti, Vincenzo
    Cantoni, Silvia
    Cavallini, Claudia
    Bianchi, Francesca
    Valente, Sabrina
    Frascari, Irene
    Olivi, Elena
    Aquaro, Giovanni D.
    Bonavita, Francesca
    Scarlata, Ignazio
    Maioli, Margherita
    Vaccari, Valentina
    Tassinari, Riccardo
    Bartoli, Antonietta
    Recchia, Fabio A.
    Pasquinelli, Gianandrea
    Ventura, Carlo
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2010, 285 (13) : 9949 - 9961
  • [32] EARLY AUTOLOGOUS STEM CELL TRANSPLANTATION IMPROVES SURVIVAL IN NEWLY DIAGNOSED MULTIPLE MYELOMA PATIENTS
    Cavallo, F.
    Spencer, A.
    Gay, F.
    Hajek, R.
    Petrucci, M. T.
    Ben Yehuda, D.
    Carella, A. M.
    Vallone, L. M.
    Oliva, S.
    Petro, D.
    Crippa, C.
    Siniscalchi, A.
    Gilestro, M.
    Pour, L.
    Maracci, L.
    Pescosta, N.
    Liberati, M.
    Magarotto, V.
    Scudla, V.
    Benevolo, G.
    Pulini, S.
    Caravita, T.
    Hardan, I.
    Boccadoro, M.
    Palumbo, A.
    HAEMATOLOGICA, 2014, 99 : 520 - 520
  • [33] Enhancement of Megakaryocyte Interactions with the Osteoblastic Hematopoietic Stem Cell Niche Improves Engraftment Efficiency Following Hematopoietic Stem Cell Transplantation
    Olson, Timothy S.
    Otsuru, Satoru
    Hofmann, Ted
    Horwitz, Edwin M.
    BLOOD, 2012, 120 (21)
  • [34] Progenitor and lymphoma cells in blood stem cell harvests: impact on survival following transplantation
    A Demirkazik
    A Kessinger
    JO Armitage
    PJ Bierman
    J Lynch
    J Vose
    W Chan
    JG Sharp
    Bone Marrow Transplantation, 2001, 28 : 207 - 212
  • [35] Survival of patients who develop solid tumors following hematopoietic stem cell transplantation
    Ehrhardt, M. J.
    Brazauskas, R.
    He, W.
    Rizzo, J. D.
    Shaw, B. E.
    BONE MARROW TRANSPLANTATION, 2016, 51 (01) : 83 - 88
  • [36] SURVIVAL OF PATIENTS WHO DEVELOP SOLID TUMORS FOLLOWING HEMATOPOIETIC STEM CELL TRANSPLANTATION
    Ehrhardt, Matthew J.
    Brazauskas, Ruta
    He, Wensheng
    Rizzo, J. Douglas
    Shaw, Bronwen E.
    PEDIATRIC BLOOD & CANCER, 2015, 62 : 108 - 109
  • [37] The impact of HLA genotyping on survival following unrelated donor haematopoietic stem cell transplantation
    Shaw, Bronwen E.
    Arguello, Rafael
    Garcia-Sepulveda, Christian A.
    Madrigal, J. Alejandro
    BRITISH JOURNAL OF HAEMATOLOGY, 2010, 150 (03) : 251 - 258
  • [38] Survival of patients who develop solid tumors following hematopoietic stem cell transplantation
    M J Ehrhardt
    R Brazauskas
    W He
    J D Rizzo
    B E Shaw
    Bone Marrow Transplantation, 2016, 51 : 83 - 88
  • [39] Progenitor and lymphoma cells in blood stem cell harvests: impact on survival following transplantation
    Demirkazik, A
    Kessinger, A
    Armitage, JO
    Bierman, PJ
    Lynch, J
    Vose, J
    Chan, W
    Sharp, JG
    BONE MARROW TRANSPLANTATION, 2001, 28 (02) : 207 - 212
  • [40] Obese Patients Have Improved Survival Following Autologous Hematopoietic Stem Cell Transplantation
    Duong, Hien K.
    Koo, Anna
    Rybicki, Lisa
    Copelan, Ed
    Kalaycio, Matt
    Sobecks, Ronald
    Dean, Robert M.
    Hill, Brian T.
    Ondrejka, Sarah L.
    Abounader, Donna M.
    Pohlman, Brad
    Bolwell, Brian J.
    BLOOD, 2012, 120 (21)
12345