Kinetic study of oily sludge biodegradation under shaking condition

In this research, the biodegrade ability of oily sludge was investigated. Two bacterial strains isolated from the oil storage tank were identified as Aneurinibacillus migulanus and Bacillus toyonensis and submitted in the gene bank database National Center for Biotechnology Information (NCBI). Three bacterial treatments consisting of identified isolates with the initial concentration of 2 g/L of the sludge were used to find the best treatment in the utilisation of the oily sludge under a shaking condition. The results of the oily sludge biodegradation with initial concentrations of 20, 50, and 100 g/L during 150 days showed 72.4, 53, and 43.9% hydrocarbon utilisation, respectively. The results of GC chromatograph showed that all hydrocarbon fractions were utilised in nearly the same range. Additionally, kinetic studies revealed that Contois model can accurately predict the oily sludge biodegradation. Monte Carlo method was used to fit the kinetic parameters of the model to experimental data.