Changes in Laminin Expression Pattern during Early Differentiation of Human Embryonic Stem Cells

Laminin isoforms laminin-511 and -521 are expressed by human embryonic stem cells (hESC) and can be used as a growth matrix to culture these cells under pluripotent conditions. However, the expression of these laminins during the induction of hESC differentiation has not been studied in detail. Furthermore, the data regarding the expression pattern of laminin chains in differentiating hESC is scarce. In the current study we aimed to fill this gap and investigated the potential changes in laminin expression during early hESC differentiation induced by retinoic acid (RA). We found that laminin-511 but not -521 accumulates in the committed cells during early steps of hESC differentiation. We also performed a comprehensive analysis of the laminin chain repertoire and found that pluripotent hESC express a more diverse range of laminin chains than shown previously. In particular, we provide the evidence that in addition to alpha 1, alpha 5, beta 1, beta 2 and gamma 1 chains, hESC express alpha 2, alpha 3, beta 3, gamma 2 and gamma 3 chain proteins and mRNA. Additionally, we found that a variant of laminin alpha 3 chain-145 kDa-accumulated in RA-treated hESC showing that these cells produce prevalently specifically modified version of alpha 3 chain in early phase of differentiation.