MULTI-SPECTRAL TUNABLE EXCITATION FLUORESCENCE MICROSCOPY WITH A NANOIMPRINTED PDMS-ON-SILICON GRATING OPTICAL FILTER

被引：0

作者：

Huang, N. -T. ^{[1
]}

Truxal, S. ^{[1
]}

Tung, Y. -C. ^{[1
]}

Kurabayashi, K. ^{[1
]}

机构：

[1] Univ Michigan, Ann Arbor, MI 48109 USA

来源：

2012 IEEE 25TH INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS) | 2012年

关键词：

SINGLE MOLECULES;

D O I：

暂无

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

We report a MEMS-based tunable grating optical filter device and its integration with a fluorescence microscope system. This integration allows for voltage-controlled wavelength tuning of excitation light from a multi-band source. We demonstrate the system's capability to selectively excite fluorescence protein molecules with different excitation/emission spectral characteristics using a chip of optical filter device arrays, each with a very small (2mm x 2 mm) footprint. The demonstrated technology has potential to enable massively-parallel multispectral fluorescence microscopy in biochip array settings.

引用

页数：4

共 21 条

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[10] Blind Decomposition of Multi-spectral Fluorescence Lifetime Imaging Microscopy Data: Further Validation
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