Isolation and Molecular Characterization of Toxoplasma gondii from Chickens in China

被引：15
|
作者：
Zhao Guang-wei [1 ]
Shen Bo [1 ]
Xie Qing [1 ]
Xu Li-xin [1 ]
Yan Ruo-feng [1 ]
Song Xiao-kai [1 ]
Adam, Hassan Ibrahim [1 ]
Li Xiang-rui [1 ]
机构：
[1] Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Jiangsu, Peoples R China
来源：
JOURNAL OF INTEGRATIVE AGRICULTURE | 2012年 / 11卷 / 08期
关键词：
Toxoplasma gondii; chickens; China; isolation; FREE-RANGE CHICKENS; BIOLOGIC CHARACTERISTICS; UNITED-STATES; SOUTH-AMERICA; STRAINS; INFECTION; BRAZIL;
D O I：
10.1016/S2095-3119(12)60133-6
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
One strain of Toxoplasma gondii was successfully isolated from chickens in China by bioassay in mice. Antibodies and circulating antigens of T. gondii were assayed by the ELISA kits in 100 free range chickens from a rural area surrounding Funing, China. Fifty-three chickens were antibody-positive and 21 chickens were antigen positive. Hearts, brains, spleens, lungs, livers, and kidneys of 21 antibody or antigen-positive chickens were bioassayed in mice. One strain of T. gondii was isolated from 1 of 21 (4.76%) chickens. The isolated T. gondii killed all of the inoculated mice. Genotyping of this isolate using polymorphisms at the loci 5'-SAG2, 3'-SAG2, SAG3, cB21-4, L358, BTUB, and GRA6 revealed that it was Type I. These indicated that it was virulent for mice. This is the first report of isolation of T gondii from chickens in China.
引用
收藏
页码：1347 / 1353
页数：7
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