Molecular Diversity Analysis of Selected Drought Resistant Chickpea (Cicer arietinum L) Genotypes

被引：0
|
作者：
Rashmi [1 ]
Singh, Rajesh Kumar [1 ]
Prerna [1 ]
Senger, R. S. [1 ]
Bhatnagar, S. K. [2 ]
Kumar, Rajendra [1 ]
机构：
[1] Sardar Vallabhbhai Patel Univ Agr & Technol, Coll Agr, Dept Agr Biotechnol, Meerut 250110, Uttar Pradesh, India
[2] Sardar Vallabhbhai Patel Univ Agr & Technol, Coll Biotechnol, Dept Cell Biol, Meerut 250110, Uttar Pradesh, India
来源：
VEGETOS | 2012年 / 25卷 / 01期
关键词：
Chickpea; microsatellite marker; molecular diversity; PIC; SSR; TAGGED MICROSATELLITE SITES; GENETIC-VARIABILITY; MARKERS;
D O I：
暂无
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Molecular characterization of drought resistant germplasm helps in identification of genetically diverse germplasm lines for crop improvement and in mapping the traits. Present paper deals with molecular characterization of 8 particular abiotic (drought) resistant genotypes using a specific set of 35 polymorphic SSR (Simple Sequence Repeat) markers for the first time. The number of alleles ranged from 1 to 3 per locus. The PIC (Polymorphism Information Content) value ranged from 0 to 0.55 with an average of 0.26 indicating the considerable efficiency of markers for studying the polymorphism level. The primer 8 showed maximum PIC value (0.553), while the lowest PIC value 0.000 was observed in ten primers. The dendrogram derived from this analysis has two main clusters. The maximum similarity with a coefficient value (0.952) was found between two pairs. The conservation of these drought resistant genotype is recommended for their utilization in Middle East Asia region chickpea improvement programs.
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收藏
页码：111 / 116
页数：6
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