Apoptotic effect of green synthesized gold nanoparticles from Curcuma wenyujin extract against human renal cell carcinoma A498 cells

被引:80
|
作者
Liu, Rui [1 ]
Pei, Qiang [2 ]
Shou, Tao [1 ]
Zhang, Wenjing [1 ]
Hu, Jing [1 ]
Li, Wei [3 ]
机构
[1] First Peoples Hosp Yunnan Prov, Dept Oncol, Kunming 650032, Yunnan, Peoples R China
[2] First Peoples Hosp Yunnan Prov, Dept Hematol, Kunming 650032, Yunnan, Peoples R China
[3] First Peoples Hosp Yunnan Prov, Dept Urinary Surg, Kunming 650032, Yunnan, Peoples R China
来源
INTERNATIONAL JOURNAL OF NANOMEDICINE | 2019年 / 14卷
关键词
Curcuma wenyujin; CWAuNPs; renal cancer; A498; anticancer agent; apoptosis; BREAST-CANCER CELLS; PHYTOMEDICINE; DELIVERY; THERAPY; GROWTH; DEATH;
D O I
10.2147/IJN.S203222
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
Introduction: Curcuma wenyujin is a plant which belongs to the family of Zingiberaceae, found in South Asia and China. C. wenyujin is a major constituent in Chinese traditional medicine and is used to treat liver diseases, blood clots, and is also prescribed as a painkiller. C. wenyujin possesses antioxidant, antiproliferative, and antitumorogenic properties, and many researchers have proved the efficacy of C. wenyujin against various types of cancer. The major drawback of this historical drug is its low bioavailability. Methods: This study synthesized gold nanoparticles using C. wenyujin and assessed its potency against in vitro renal cancer cells. The biosynthesized C. wenyujin gold nanoparticles (CWAuNPs) were characterized using UV-Spec, DLS, FTIR, SAED, TEM, EDAX, and Atomic Force analysis. The cytotoxicity of CWAuNPs against renal cancer cell lines A498 and SW-156 was assessed with MTT assay. The induction of apoptosis by CWAuNPs in A498 cell was measured using apoptotic staining DAPI, Rhodamine 123, and H2DCFDA. The apoptotic activity of CWAuNPs was further confirmed with flow cytometric analysis. The molecular mechanism of CWAuNPs was analyzed with qPCR and immunoblotting analysis of caspases, proapoptotic, and antiapoptotic proteins. Results: The characterization of results of synthesized CWAuNPs satisfy the distinctive properties of a potent nanodrug. The results of apoptotic staining techniques confirm the induction of CWAuNPs in A498 by increasing the apoptotic Caspase 3,9, Bid, and Bad, and decreasing the antiapoptotic protein Bcl-2, Bcl-xl expressions, which is authentically proven by the qPCR and immunoblotting analysis. Conclusion: In conclusion, these results confirmed that biosynthesized CWAuNPs is a potent anticancer agent which induces apoptosis in the A498 renal carcinoma cell line.
引用
收藏
页码:4091 / 4103
页数:13
相关论文
共 50 条
  • [21] Metformin-induced apoptosis facilitates degradation of the cellular caspase 8 (FLICE)-like inhibitory protein through a caspase-dependent pathway in human renal cell carcinoma A498 cells
    Jang, Ji-Hoon
    Song, In-Hwan
    Sung, Eon-Gi
    Lee, Tae-Jin
    Kim, Joo-Young
    ONCOLOGY LETTERS, 2018, 16 (02) : 2030 - 2038
  • [22] Green tea extract mediated biogenic synthesis of gold nanoparticles with potent anti-proliferative effect against PC-3 human prostate cancer cells
    Prema, P.
    Boobalan, T.
    Arun, A.
    Rameshkumar, K.
    Babu, R. Suresh
    Veeramanikandan, V.
    Van-Huy Nguyen
    Balaji, P.
    MATERIALS LETTERS, 2022, 306
  • [23] Gold Nanoparticles Synthesized with a Polyphenols-Rich Extract from Cornelian Cherry (Cornus mas) Fruits: Effects on Human Skin Cells
    Perde-Schrepler, Maria
    David, Luminita
    Olenic, Liliana
    Potara, Monica
    Fischer-Fodor, Eva
    Virag, Piroska
    Imre-Lucaci, Florica
    Brie, Ioana
    Florea, Adrian
    JOURNAL OF NANOMATERIALS, 2016, 2016
  • [24] Cytotoxic and Apoptotic Effect of Rubus chingii Leaf Extract against Non-Small Cell Lung Carcinoma A549 Cells
    Khafagy, El-Sayed
    Al Saqr, Ahmed
    Alotaibi, Hadil Faris
    Abu Lila, Amr Selim
    PROCESSES, 2022, 10 (08)
  • [25] An investigation on the cytotoxicity and caspase-mediated apoptotic effect of biologically synthesized silver nanoparticles using Podophyllum hexandrum on human cervical carcinoma cells
    Jeyaraj, Murugaraj
    Rajesh, Manoharan
    Arun, Renganathan
    MubarakAli, Davoodbasha
    Sathishkumar, Gnanasekar
    Sivanandhan, Ganeshan
    Dev, Gnanajothi Kapil
    Manickavasagam, Markandan
    Premkumar, Kumpati
    Thajuddin, Nooruddin
    Ganapathi, Andy
    COLLOIDS AND SURFACES B-BIOINTERFACES, 2013, 102 : 708 - 717
  • [26] Impact of Green-synthesized Copper Silver Nanoparticles Against Human Colon Cancer Cells Utilizing Sargassum muticum Extract in Terms of Cytotoxicity and Apoptosis
    Almutairi, Bader O.
    Ali, Daoud
    Almarzoug, Mohammed H. A.
    Asiri, Atheer M.
    Alkahtani, Saad
    Alarifi, Saud
    NATURAL PRODUCT COMMUNICATIONS, 2024, 19 (11)
  • [27] Effect of petroleum ether extract of Panax ginseng roots on proliferation and cell cycle progression of human renal cell carcinoma cells
    Jeongwon Sohn
    Chul-Hee Lee
    Dong Jun Chung
    Sul Hee Park
    Insun Kim
    Woo Ik Hwang
    Experimental & Molecular Medicine, 1998, 30 : 47 - 51
  • [28] Effect of petroleum ether extract of Panax ginseng roots on proliferation and cell cycle progression of human renal cell carcinoma cells
    Sohn, J
    Lee, CH
    Chung, DJ
    Park, SH
    Kim, I
    Hwang, WI
    EXPERIMENTAL AND MOLECULAR MEDICINE, 1998, 30 (01): : 47 - 51
  • [29] Apoptotic Effect and Anticancer Activity of Biosynthesized Silver Nanoparticles from Marine Algae Chaetomorpha linum Extract Against Human Colon Cancer Cell HCT-116
    Diptikanta Acharya
    Sagarika Satapathy
    Prathap Somu
    Umesh Kumar Parida
    Gitanjali Mishra
    Biological Trace Element Research, 2021, 199 : 1812 - 1822
  • [30] Ag-doped ZnO nanoparticles synthesized through green method using Artemisia turcomanica extract induce cytotoxicity and apoptotic activities against AGS cancer cells: an in vitro study
    Rad, Fatemeh Mirzaee
    Tafvizi, Farzaneh
    Noorbazargan, Hassan
    Iranbakhsh, Alireza
    JOURNAL OF NANOSTRUCTURE IN CHEMISTRY, 2024, 14 (06) : 403 - 418